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High Acetone-Butanol-Ethanol Production from Food Waste by Recombinant Clostridium saccharoperbutylacetonicum in Batch and Continuous Immobilized-Cell Fermentation
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-06-08 , DOI: 10.1021/acssuschemeng.0c02529 Qing Jin 1 , Zhaohui An 2 , Ashok Damle 3 , Nicholas Poe 1 , Jian Wu 1 , Hengjian Wang 1 , Zhiwu Wang 2 , Haibo Huang 1
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-06-08 , DOI: 10.1021/acssuschemeng.0c02529 Qing Jin 1 , Zhaohui An 2 , Ashok Damle 3 , Nicholas Poe 1 , Jian Wu 1 , Hengjian Wang 1 , Zhiwu Wang 2 , Haibo Huang 1
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Sustainable and economical production of butanol via acetone-butanol-ethanol (ABE) fermentation faces several major challenges, including high feedstock cost, low butanol yield, and low butanol productivity. To address these challenges, low-cost food waste was used as feedstock for ABE production in batch and continuous immobilized-cell fermentation by a recombinant high-butanol production strain Clostridium saccharoperbutylacetonicum deltptabuk. Food waste was first liquefied and saccharified to obtain fermentable sugars. After that, food waste hydrolysates were fed into both batch and continuous immobilized-cell fermentation systems to produce ABE. In the batch fermentation, only 14.32 g/L ABE was produced using food waste hydrolysates medium. However, when food waste hydrolysate medium was fed into the continuous immobilized-cell fermentation, remarkable increases of ABE production, yield, and productivity were achieved. At the dilution rate of 0.1 h–1, 19.65 g/L ABE was produced with an ABE yield of 0.43. At the dilution rate of 0.39 h–1, the highest ABE productivity (4.56 g/L/h) was obtained, which was 23 times higher than that in the batch fermentation. This study for the first time demonstrated efficient conversion of food waste to butanol via continuous immobilized-cell fermentation to achieve high titer and productivity, which would potentially support the advanced utilization of organic waste materials for biofuel production.
中文翻译：
分批重组连续糖胞发酵和固定化发酵从食物垃圾中大量生产丙酮-丁醇-乙醇
通过丙酮-丁醇-乙醇（ABE）发酵可持续且经济地生产丁醇面临数项主要挑战，包括原料成本高，丁醇收率低和丁醇生产率低。为了应对这些挑战，低成本的食品垃圾被用作重组高丁醇生产菌株梭状芽孢梭菌（Clostridium saccharoperbutylacetonicum deltptabuk）批量生产和连续固定细胞发酵的原料，用于ABE生产。首先将食物垃圾液化并糖化以获得可发酵的糖。之后，将食物残渣水解产物送入分批和连续固定细胞发酵系统中以生产ABE。在分批发酵中，使用食物残渣水解产物培养基仅生产14.32 g / L ABE。然而，当将食物废水解产物培养基加入到连续的固定细胞发酵中时，ABE的产量，产量和生产率得到了显着提高。在0.1 h –1的稀释率下，生产的ABE为19.65 g / L，ABE的产率为0.43。稀释率为0.39 h –1，获得了最高的ABE生产率（4.56 g / L / h），是分批发酵的23倍。这项研究首次证明，通过连续的固定化细胞发酵，可将食物残渣有效地转化为丁醇，从而实现高滴定度和生产率，这有可能支持有机废物原料在生物燃料生产中的先进利用。
更新日期：2020-07-06
中文翻译：
分批重组连续糖胞发酵和固定化发酵从食物垃圾中大量生产丙酮-丁醇-乙醇
通过丙酮-丁醇-乙醇（ABE）发酵可持续且经济地生产丁醇面临数项主要挑战，包括原料成本高，丁醇收率低和丁醇生产率低。为了应对这些挑战，低成本的食品垃圾被用作重组高丁醇生产菌株梭状芽孢梭菌（Clostridium saccharoperbutylacetonicum deltptabuk）批量生产和连续固定细胞发酵的原料，用于ABE生产。首先将食物垃圾液化并糖化以获得可发酵的糖。之后，将食物残渣水解产物送入分批和连续固定细胞发酵系统中以生产ABE。在分批发酵中，使用食物残渣水解产物培养基仅生产14.32 g / L ABE。然而，当将食物废水解产物培养基加入到连续的固定细胞发酵中时，ABE的产量，产量和生产率得到了显着提高。在0.1 h –1的稀释率下，生产的ABE为19.65 g / L，ABE的产率为0.43。稀释率为0.39 h –1，获得了最高的ABE生产率（4.56 g / L / h），是分批发酵的23倍。这项研究首次证明，通过连续的固定化细胞发酵，可将食物残渣有效地转化为丁醇，从而实现高滴定度和生产率，这有可能支持有机废物原料在生物燃料生产中的先进利用。
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