Metal ions and anions play significant roles in biological systems and industrial processes, therefore it is important to develop good fluorescent probes to detect metal ions and anions. Here, N,O‐co‐doped carbon quantum dots (CDs) that could detect Zn²⁺ via a ratiometric fluorescence method were fabricated. The reaction between the as‐prepared CDs and zinc acetate gave the composite CDs–Zn, in which fluorescence changed ratiometrically upon addition of S₂O₈²⁻. With change in excitation light, the emission peaks of the CDs and CDs–Zn were kept fixed while intensity changed. CDs and CDs–Zn exhibited good photostability, thermal stability, selectivity, and strong anti‐interference ability. In addition, CDs and CDs–Zn displayed low dark toxicity under physiological temperatures. Ratiometric fluorescence imaging of intracellular Zn²⁺ and S₂O₈²⁻ was carried out in living HeLa cells for both of these probes. Compared with reported ratiometric fluorescent hybrid nanosensors based on organic dyes and inorganic nanomaterials, the as‐prepared CDs and CDs–Zn had low toxicity, and were easy to synthesize.

中文翻译：

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基于碳点的比例式荧光探针，具有低暗毒性的细胞内锌离子和过硫酸根离子。

金属离子和阴离子在生物系统和工业过程中起着重要作用，因此开发出良好的荧光探针来检测金属离子和阴离子非常重要。在这里，制造了可以通过比例荧光方法检测Zn ^2+的N，O共掺杂碳量子点（CD）。制备的CD与乙酸锌之间的反应产生了复合CD-Zn，其中添加S ₂ O ₈ ^2-时荧光成比例地变化。随着激发光的变化，CDs和CDs-Zn的发射峰保持固定，而强度发生变化。CD和CD-Zn具有良好的光稳定性，热稳定性，选择性和强大的抗干扰能力。此外，CD和CD-Zn在生理温度下显示出较低的暗毒性。对于这两种探针，都在活HeLa细胞中进行了细胞内Zn ²⁺和S ₂ O ₈ ^2-的比例荧光成像。与已报道的基于有机染料和无机纳米材料的比例荧光混合纳米传感器相比，所制备的CD和CD-Zn具有较低的毒性，并且易于合成。