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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.
Nature Communications ( IF 14.7 ) Pub Date : 2016-07-06 , DOI: 10.1038/ncomms12119
Carsten Rautengarten , Berit Ebert , Lifeng Liu , Solomon Stonebloom , Andreia M. Smith-Moritz , Markus Pauly , Ariel Orellana , Henrik Vibe Scheller , Joshua L. Heazlewood
Nature Communications ( IF 14.7 ) Pub Date : 2016-07-06 , DOI: 10.1038/ncomms12119
Carsten Rautengarten , Berit Ebert , Lifeng Liu , Solomon Stonebloom , Andreia M. Smith-Moritz , Markus Pauly , Ariel Orellana , Henrik Vibe Scheller , Joshua L. Heazlewood
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Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
中文翻译:
植物发育需要拟南芥高尔基本地化的GDP-L-岩藻糖转运蛋白。
跨越高尔基体膜的核苷酸糖转运对于聚糖结构的腔内生物合成是必不可少的。在这里,我们确定了GDP-岩藻糖转运蛋白1(GFT1),一种拟南芥核苷酸糖转运蛋白,它将GDP-L-岩藻糖转移到高尔基体腔中。使用基于蛋白脂质体的转运测定法,我们显示GFT在体外比其他核苷酸糖优先转运GDP-L-岩藻糖,而GFT1沉默的植物在细胞壁来源的木葡聚糖和鼠李糖半乳糖醛酸聚糖II中几乎不含L-岩藻糖。此外,这些品系显示出N-聚糖结构中L-岩藻糖含量降低,并伴有严重的发育生长缺陷。我们得出的结论是,GFT1是将GDP-L-岩藻糖进口到高尔基体的主要核苷酸糖转运体,是植物正常生长和发育所必需的。
更新日期:2016-07-09
中文翻译:
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植物发育需要拟南芥高尔基本地化的GDP-L-岩藻糖转运蛋白。
跨越高尔基体膜的核苷酸糖转运对于聚糖结构的腔内生物合成是必不可少的。在这里,我们确定了GDP-岩藻糖转运蛋白1(GFT1),一种拟南芥核苷酸糖转运蛋白,它将GDP-L-岩藻糖转移到高尔基体腔中。使用基于蛋白脂质体的转运测定法,我们显示GFT在体外比其他核苷酸糖优先转运GDP-L-岩藻糖,而GFT1沉默的植物在细胞壁来源的木葡聚糖和鼠李糖半乳糖醛酸聚糖II中几乎不含L-岩藻糖。此外,这些品系显示出N-聚糖结构中L-岩藻糖含量降低,并伴有严重的发育生长缺陷。我们得出的结论是,GFT1是将GDP-L-岩藻糖进口到高尔基体的主要核苷酸糖转运体,是植物正常生长和发育所必需的。