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A mitochondrion-targeting fluorescent probe for hypochlorite anion in living cells.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.saa.2020.118564
Wenjing Liu 1 , Yuying Wang 1 , Na Wu 2 , Wei Feng 2 , Zhanxian Li 1 , Liuhe Wei 1 , Mingming Yu 1
Affiliation  

As momentous reactive oxygen species (ROS), it is necessary to develop high-sensitivity and high-specificity fluorescent probes for tracking hypochlorite anion (ClO) in environmental and biological systems. Herein, a kind of red luminescent carbon dots (NS-dots) was synthesized by one-step solvothermal method to detect ClO in PBS buffer solution (VPBS:VEtOH = 100:1, pH = 7.4). The NS-dots has high sensitivity and low detection limit (13.3 μmol/L) for detecting ClO with linear range from 6.7 × 10−5 mol/L to 26.7 × 10−5 mol/L. Using Rhodamine B (31% at 520 nm in water) as a reference, the NS-dots have a fluorescence quantum yield of 7.2%. Intracellular photostability, mitochondrial targeting properties and the fluorescence imaging towards intracellular ClO were demonstrated.



中文翻译:

用于活细胞中次氯酸根阴离子的线粒体靶向荧光探针。

作为重大的活性氧物质(ROS),有必要开发高灵敏度和高特异性荧光探针用于跟踪次氯酸阴离子(CLO - )对环境和生物系统。在本文中,一种红色发光碳点(NS-点)通过一步法溶剂热法合成来检测CLO -在PBS缓冲液溶液(V PBS:V乙醇 = 100:1,pH值= 7.4)。对NS-点具有用于检测CLO高灵敏度和低检测限(13.3微摩尔/ L)-的线性范围为6.7×10 -5 摩尔/ L至26.7×10 -5 摩尔/升。使用若丹明B(在水中520 nm时为31%)作为参考,NS点的荧光量子产率为7.2%。细胞内的光稳定性,线粒体靶向性能和朝内的CLO荧光成像-进行了论证。

更新日期:2020-06-01
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