Dahuang zhechong pill (DHZCP) is a famous traditional Chinese medicine prescription, which is widely used in the treatment of liver diseases. However, due to the lack of a dynamic DHZCP profile, the in vivo pharmacokinetics of active ingredients within this medicine remains unknown. In this paper, a rapid, sensitive and reliable UHPLC-MS/MS method was used to determine the content of 19 characteristic constituents of DHZCP in rat plasma, including rhein, emodin, chrysophanol, physcion, aloeemodin, p-methoxyphenylacetic acid, hypoxanthine nucleoside, wogonin, wogonoside,baicalin, norwogonin, naringenin, nutmeg acid, paeoniflorin, verbascoside, rhodiola glucoside, forsythoside A, formononetin, and glycyrrhizic acid. An Agilent Extend-C₁₈ column (2.1 mm × 100 mm, 1.8 μm) was used to separate the 19 characteristic constituents, with a mobile phrase of (A) 0.1% formic acid and (B) acetonitrile. The constituents were detected in negative ion mode with multiple reactions monitoring (MRM). The established UHPLC-MS/MS method had good linearity, with a coefficient of determination (r²) of >0.99. The daytime and intra-day precision were less than 12%, and the accuracy ranged from -9.56% to 7.82%. The stability, extraction recovery, and matrix effect met the requirements. The method was successfully applied to the pharmacokinetic study of these nineteen characteristic constituents after oral administration of DHZCP. UHPLC-MS/MS was used for the first time to study the pharmacokinetics of the characteristic chemical constituents in DHZCP, which provided reference and theoretical guidance for further clarification of its pharmacodynamic basis.

大黄遮虫丸（DHZCP）是著名的中药方剂，广泛用于治疗肝脏疾病。然而，由于缺乏动态 DHZCP 谱，该药物中活性成分的体内药代动力学仍然未知。本文采用快速、灵敏、可靠的UHPLC-MS/MS方法测定了大鼠血浆中DHZCP中大黄酸、大黄素、大黄酚、大黄素甲醚、芦荟大黄素、对甲氧基苯乙酸、次黄嘌呤核苷等19种特征成分的含量。 、汉黄芩素、汉黄芩苷、黄芩苷、降汉黄芩素、柚皮素、肉豆蔻酸、芍药苷、毛蕊花苷、红景天苷、连翘苷A、芒柄花素和甘草酸。使用 Agilent Extend-C ₁₈色谱柱（2.1 mm × 100 mm，1.8 μm）分离 19 种特征成分，流动相为 (A) 0.1% 甲酸和 (B) 乙腈。通过多重反应监测 (MRM) 以负离子模式检测成分。建立的UHPLC-MS/MS方法线性良好，测定系数(r ² )为>0.99。日间和日内精度均小于12%，准确度范围为-9.56%~7.82%。稳定性、提取回收率、基质效应均满足要求。该方法成功应用于DHZCP口服后这19种特征成分的药动学研究。首次采用UHPLC-MS/MS研究DHZ​​CP中特征化学成分的药代动力学，为进一步阐明其药效基础提供参考和理论指导。