A method was developed that used hydroxylamine hydrochloride as a probe in a superoxide anion radical-generating visible light system for determining superoxide anion radicals. An Azure I solution with hydroxylamine hydrochloride was illuminated, after which a ferric iron solution was added to the sample solution and the remaining hydroxylamine hydrochloride in solution reduced from ferric to ferrous ions. Then, 1,10-phenanthroline solution was added and spectrophotometrically measured at 510 nm, which indirectly indicated the hydroxylamine hydrochloride content. The yields of superoxide anion radicals were indirectly expressed by the hydroxylamine hydrochloride decrement. Under optimal experimental conditions, the linear range was 0.0–1.5 × 10⁻⁵ M and the limit of detection and limit of quantification were obtained to be 8.37 × 10⁻⁷ and 2.54 × 10⁻⁶ M with an R² of 0.9993. The method was simple and feasible and could be used for the stable measurement of superoxide anion radicals produced by photosensitizers that produce color under acidic conditions in visible light systems.

开发了一种方法，该方法使用羟胺盐酸盐作为产生超氧阴离子自由基的可见光系统中的探针，以确定超氧阴离子自由基。照亮含有盐酸羟胺的Azure I溶液，然后将三价铁溶液添加到样品溶液中，并将溶液中剩余的盐酸羟胺从三价铁离子还原为亚铁离子。然后，加入1,10-菲咯啉溶液并在510 nm处进行分光光度法测定，间接表明盐酸羟胺的含量。超氧阴离子自由基的产率通过盐酸羟胺减量间接表示。在最佳实验条件下，线性范围为0.0–1.5×10 ^-5 M以及检测极限和定量极限为8.37×10 ^-7和2.54×10 ^-6  M，R ²为0.9993。该方法简单可行，可用于稳定测量由光敏剂产生的超氧阴离子自由基，该光敏剂在酸性条件下在可见光系统中产生颜色。