Downy mildew, caused by Plasmopara viticola, is a major disease that affects grapevines, and a few resistance (R) genes have been identified thus far. In order to identify R genes, we investigated F₁ progeny from a cross between the downy mildew-resistant Vitis amurensis ‘Shuang Hong’ and the susceptible Vitis vinifera ‘Cabernet Sauvignon’. The P. viticola-resistance of the progeny varied continuously and was segregated as a quantitative trait. Genotyping-by-sequencing was used to construct linkage maps. The integrated map spanned 1898.09 cM and included 5603 single nucleotide polymorphisms on 19 linkage groups (LGs). Linkage analysis identified three quantitative trait loci (QTLs) for P. viticola resistance: 22 (Rpv22) on LG 02, Rpv23 on LG15, and Rpv24 on LG18. The phenotypic variance contributed by these three QTLs ranged from 15.9 to 30.0%. qRT-PCR analysis of R-gene expression in these QTLs revealed a CC-NBS-LRR disease resistance gene RPP8, two LRR receptor-like serine/threonine-protein kinases, a serine/threonine-protein kinase BLUS1, a glutathione peroxidase 8, an ethylene-responsive transcription factor ERF038, and a transcription factor bZIP11 were induced by P. viticola, and these genes may play important role in P. viticola response.

霜霉病，造成葡萄霜霉病，是一个重大的疾病，影响葡萄树，和几个电阻（[R ）基因，迄今已确定。为了鉴定R基因，我们从耐霜霉的葡萄“双虹”和易感的葡萄“赤霞珠”之间的杂交中研究了F ₁后代。该P.霜霉病后代的抗性不断变化，并被隔离为数量性状。通过测序进行基因分型来构建连锁图。整合图跨越了1890.99 cM，在19个连锁基团（LG）上包括5603个单核苷酸多态性。连锁分析确定了对葡萄黄单胞菌抗性的三个定量性状基因座（QTL）：LG 02上为22（Rpv22），LG15上为Rpv23，LG18上为Rpv24。这三个QTL贡献的表型差异范围为15.9％至30.0％。这些QTL中R基因表达的qRT-PCR分析显示CC-NBS-LRR疾病抗性基因RPP8中，两个LRR受体样丝氨酸/苏氨酸-蛋白激酶，丝氨酸/苏氨酸-蛋白激酶BLUS1，谷胱甘肽过氧化物酶8，乙烯-响应转录因子ERF038，和转录因子bZIP11诱导由P.葡萄生单轴霉，和这些基因可能在葡萄球菌的反应中起重要作用。