<br>鞘糖脂质谱分析的最新进展——综述,Analytica Chimica Acta

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鞘糖脂质谱分析的最新进展——综述
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.aca.2020.05.051
Rodell C Barrientos ₁ , Qibin Zhang ₁

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鞘糖脂的异常表达与多种疾病有关，但我们对此类生物分子的结构多样性、空间分布和生物学功能的了解仍然有限。这些挑战部分源于缺乏能够在分子水平上检测、识别和量化鞘糖脂的敏感工具。质谱分析已成为解决大部分挑战的强大工具。在这里，我们回顾了分析鞘糖脂组学的最新进展，重点是样品制备、质谱和基于串联质谱的结构表征、无标记和基于标记的定量。我们还讨论了鞘糖脂的命名法，以及区分鞘糖脂异构体的离子迁移谱等新兴技术。根据个人的研究目标，对每种方法的内在优点和缺点进行了仔细的批评。最后，阐述了分析鞘脂组学的未来前景，包括在异构体分离、低丰度物种检测以及使用成像质谱分析细胞和组织中鞘糖脂分子物种的空间分布方面需要新颖且更灵敏的方法。

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Recent advances in the mass spectrometric analysis of glycosphingolipidome – A review

Aberrant expression of glycosphingolipids has been implicated in a myriad of diseases, but our understanding of the strucural diversity, spatial distribution, and biological function of this class of biomolecules remains limited. These challenges partly stem from a lack of sensitive tools that can detect, identify, and quantify glycosphingolipids at the molecular level. Mass spectrometry has emerged as a powerful tool poised to address most of these challenges. Here, we review the recent developments in analytical glycosphingolipidomics with an emphasis on sample preparation, mass spectrometry and tandem mass spectrometry-based structural characterization, label-free and labeling-based quantification. We also discuss the nomenclature of glycosphingolipids, and emerging technologies like ion mobility spectrometry in differentiation of glycosphingolipid isomers. The intrinsic advantages and shortcomings of each method are carefully critiqued in line with an individual's research goals. Finally, future perspectives on analytical sphingolipidomics are stated, including a need for novel and more sensive methods in isomer separation, low abundance species detection, and profiling the spatial distribution of glycosphingolipid molecular species in cells and tissues using imaging mass spectrometry.

更新日期：2020-10-01

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