Gold nanozyme as an excellent co-catalyst for enhancing the performance of a colorimetric and photothermal bioassay,Analytica Chimica Acta

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Gold nanozyme as an excellent co-catalyst for enhancing the performance of a colorimetric and photothermal bioassay
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.05.047
Pengli An ₁ , Xin Xue ₁ , Honghong Rao ₂ , Jingjing Wang ₁ , Min Gao ₁ , Hongqiang Wang ₁ , Mingyue Luo ₁ , Xiuhui Liu ₁ , Zhonghua Xue ₁ , Xiaoquan Lu ₁

Affiliation

Advanced oxidation processes (AOPs) have recently proposed for advancing colorimetric sensing applications, owing to their excellent performance of sensitive color readout that generated from the oxidation of chromogenic substrates like 3,3',5,5'-tetramethylbenzidine (TMB) by reactive oxygen species (ROS) of AOPs such as ·OH and ·O2- radicals. However, the efficiency of ROS generation and the related H2O2 decomposition in most AOPs is quite low especially at neutral pH, which greatly hampered the practical sensing applications of the AOPs. We herein communicated that β-cyclodextrin (β-CD)-capped gold nanoparticles (β-CD@AuNPs) can promote catalysis at neutral pH for AOP as an excellent co-catalyst. In this strategy, inorganic pyrophosphate (PPi) ions was first used to coordinate with Cu2+ and form Cu2+-PPi complex. In the presence of hydrogen peroxide, target inorganic pyrophosphatase (PPase) can hydrolyze PPi into inorganic phosphate (Pi) and release free Cu2+ simultaneously, resulting in a Cu2+-triggered Fenton-like AOP reaction. The introduced β-CD@AuNPs acts as a co-catalyst, analogous to mediators in the most co-catalyzed system, to enhance the rate-limiting step of Cu2+/Cu+ conversion in Cu2+/H2O2 Fenton-like AOP and resulting in an efficient generation of ·OH and ·O2- radicals, which further producing an intense blue color by oxidizing TMB into its oxidation product (TMBox) within a short time. Finally, this reaction system was used to simply detecting target PPase with the colorimetric and photothermal readout based on the in-situ generated TMBox indicator. More significantly, we successfully demonstrated nanozyme can serve as a co-catalyst to promote the AOP catalysis at neutral pH, and inspire other strategies to overcome the pH limitation in the AOP catalysis and expand its colorimetric and photothermometric application.

中文翻译：

金纳米酶作为一种出色的助催化剂，可提高比色和光热生物测定的性能

高级氧化过程 (AOP) 最近被提议用于推进比色传感应用，因为它们具有优异的灵敏颜色读出性能，这些读出是由活性氧氧化 3,3',5,5'-四甲基联苯胺 (TMB) 等显色底物产生的AOP 的物种 (ROS)，例如·OH 和·O2- 自由基。然而，大多数 AOP 中 ROS 生成和相关 H2O2 分解的效率非常低，尤其是在中性 pH 值下，这极大地阻碍了 AOP 的实际传感应用。我们在本文中表示，β-环糊精（β-CD）封端的金纳米粒子（β-CD@AuNPs）可以在中性 pH 值下促进 AOP 作为一种出色的助催化剂的催化作用。在该策略中，无机焦磷酸盐 (PPi) 离子首先用于与 Cu2+ 配位并形成 Cu2+-PPi 络合物。在过氧化氢存在下，目标无机焦磷酸酶 (PPase) 可将 PPi 水解为无机磷酸盐 (Pi) 并同时释放游离 Cu2+，导致 Cu2+ 引发类芬顿 AOP 反应。引入的 β-CD@AuNPs 作为助催化剂，类似于大多数共催化系统中的介体，以增强 Cu2+/H2O2 Fenton-like AOP 中 Cu2+/Cu+ 转化的限速步骤，并产生高效的·OH和·O2-自由基的产生，通过在短时间内将TMB氧化为其氧化产物（TMBox），进一步产生强烈的蓝色。最后，该反应系统用于基于原位生成的 TMBox 指示剂，通过比色和光热读数简单检测目标 PPase。更重要的是，

更新日期：2020-08-01

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