Abstract Cell therapy and tissue engineering have been intensively researched for repair of articular cartilage. In this study, we investigated the chondrogenic potential of canine adipose-derived mesenchymal stromal cells (ASCs) combined to high molecular weight hyaluronic acid (HA) in vitro, and their therapeutic effect in dogs with chronic osteoarthritis (OA) associated with bilateral hip dysplasia. Canine ASCs were characterized after conventional 2D culture or 3D culture in HA, showing adequate immunophenotype, proliferation and trilineage differentiation, as well as chondrogenesis after cultivation in HA. ASC/HA constructs were used to treat 12 dogs with OA, sequentially assigned to control, ASC and ASC/HA groups. Animals were examined for clinical, orthopedic and radiological parameters. Lameness at walk and pain on manipulation were reduced in the ASC group and mainly in the ASC/HA group. Range of motion and detection of crepitus on hip rotation and abduction improved similarly in all groups. For articular edema, muscle atrophy, Norberg angle values and radiographic analyses, there were no variations throughout the period. These results indicate that ASC/HA constructs are safe and may be an effective therapeutic tool in treating canine chronic osteoarthritis, which should be confirmed with larger studies and additional clinical parameters.

中文翻译：

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结合犬间充质基质细胞和透明质酸进行软骨修复

摘要 细胞疗法和组织工程已被广泛研究用于修复关节软骨。在这项研究中，我们在体外研究了犬脂肪来源的间充质基质细胞 (ASCs) 与高分子量透明质酸 (HA) 结合的软骨形成潜力，以及它们对患有双侧髋关节发育不良相关的慢性骨关节炎 (OA) 犬的治疗效果. 在 HA 中进行常规 2D 培养或 3D 培养后对犬 ASC 进行表征，显示出足够的免疫表型、增殖和三系分化，以及在 HA 中培养后的软骨形成。ASC/HA 构建体用于治疗 12 只患有 OA 的狗，依次分配到对照组、ASC 和 ASC/HA 组。检查动物的临床、整形外科和放射学参数。在 ASC 组中，主要是在 ASC/HA 组中，行走跛行和操作疼痛减少。在所有组中，髋部旋转和外展的运动范围和捻发音检测都有相似的改善。对于关节水肿、肌肉萎缩、诺伯格角值和影像学分析，在整个期间没有变化。这些结果表明 ASC/HA 结构是安全的，可能是治疗犬慢性骨关节炎的有效治疗工具，这应该通过更大规模的研究和额外的临床参数来证实。