Characterization of Nitrate-Dependent As(III)-Oxidizing Communities in Arsenic-Contaminated Soil and Investigation of Their Metabolic Potentials by the Combination of DNA-Stable Isotope Probing and Metagenomics.,Environmental Science & Technology

当前位置： X-MOL 学术 › Environ. Sci. Technol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization of Nitrate-Dependent As(III)-Oxidizing Communities in Arsenic-Contaminated Soil and Investigation of Their Metabolic Potentials by the Combination of DNA-Stable Isotope Probing and Metagenomics.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-05-21 , DOI: 10.1021/acs.est.0c01601
Miaomiao Zhang _{1,

2,

3} , Zhe Li _{1,

4} , Max M Häggblom ₅ , Lily Young ₆ , Zijun He _{1,

2,

3} , Fangbai Li _{1,

2,

3} , Rui Xu _{1,

2,

3} , Xiaoxu Sun _{1,

2,

3} , Weimin Sun _{1,

2,

3}

Affiliation

Arsenite (As(III)) oxidation has important environmental implications by decreasing both the mobility and toxicity of As in the environment. Microbe-mediated nitrate-dependent As(III) oxidation (NDAO) may be an important process for As(III) oxidation in anoxic environments. Our current knowledge of nitrate-dependent As(III)-oxidizing bacteria (NDAB), however, is largely based on isolates, and thus, the diversity of NDAB may be underestimated. In this study, DNA-stable isotope probing (SIP) with ¹³C-labeled NaHCO₃ as the sole carbon source, amplicon sequencing, and shotgun metagenomics were combined to identify NDAB and investigate their NDAO metabolism. As(III) oxidation was observed in the treatment amended with nitrate, while no obvious As(III) oxidation was observed without nitrate addition. The increase in the gene copies of aioA in the nitrate-amended treatment suggested that As(III) oxidation was mediated by microorganisms containing the aioA genes. Furthermore, diverse putative NDAB were identified in the As-contaminated soil cultures, such as Azoarcus, Rhodanobacter, Pseudomonas, and Burkholderiales-related bacteria. Metagenomic analysis further indicated that most of these putative NDAB contained genes for As(III) oxidation and nitrate reduction, confirming their roles in NDAO. The identification of novel putative NDAB expands current knowledge regarding the diversity of NDAB. The current study also suggests the proof of concept of using DNA-SIP to identify the slow-growing NDAB.

中文翻译：

DNA稳定同位素探测与代谢组学相结合，对砷污染土壤中依赖硝酸盐的As（III）氧化社区进行表征，并研究其代谢潜力。

砷（As（III））的氧化通过降低砷在环境中的迁移率和毒性而对环境产生重要影响。微生物介导的硝酸盐依赖性As（III）氧化（NDAO）可能是缺氧环境中As（III）氧化的重要过程。但是，我们目前对依赖硝酸盐的As（III）氧化细菌（NDAB）的了解很大程度上基于分离物，因此，NDAB的多样性可能被低估了。在这项研究中，DNA稳定同位素探测（SIP）与¹³ C标记的NaHCO ₃作为唯一的碳源，扩增子测序和shot弹枪宏基因组学相结合来鉴定NDAB并研究其NDAO代谢。在用硝酸盐改良的处理中观察到As（III）氧化，而未添加硝酸盐则未观察到明显的As（III）氧化。硝酸盐改良处理中aioA基因拷贝数的增加表明，As（III）的氧化是由含有aioA基因的微生物介导的。此外，不同的推定NDAB在作为污染土壤培养物，如鉴定固氮弧菌，Rhodanobacter，假单胞菌和与伯克霍尔德里亚斯菌有关的细菌。元基因组学分析进一步表明，这些推定的NDAB中大多数含有As（III）氧化和硝酸盐还原的基因，证实了它们在NDAO中的作用。新推定的NDAB的鉴定扩展了有关NDAB多样性的现有知识。当前的研究还提出了使用DNA-SIP识别缓慢增长的NDAB的概念证明。

更新日期：2020-05-21

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南