Despite an ever-growing number of investigations and recent advances in nanozymes and oxidative stress anti-tumor therapy, nanoparticles capable of maintaining the redox balance as natural antioxidative system in normal tissues are scarce. Herein, iron phosphates (FePOs)-based nanozyme, was synthesized via a simple hydrothermal method. We first discovered that the FePOs nanozyme has trienzyme-like activities. It is peroxidase (POD)-like in acidic tumor microenvironment and can mediate a highly efficient FePOs+H₂O₂ anti-tumor protocol via catalyzing ·OH generation from H₂O₂. Quite different from any other reported nanozymes, at physiological pH the FePOs is not only catalase (CAT)-like but also superoxide dismutase (SOD)-like. It exerts combined SOD-CAT effects on normal tissues, which is analogous to the synergistic effects of natural SOD-CAT in living organisms, and hence protects normal tissues from injuries of the FePOs+H₂O₂ protocol. Apoptosis was found to be the pathway by which cell death is induced. Notably, the FePOs+H₂O₂ protocol led to no abnormal behavior, significant weight loss or obvious adverse effects on normal tissues in mice as the results of the combined SOD-CAT effects. All these intriguing results confirm that the FePOs nanozyme is an excellent candidate for nanomedicine and holds promise for enhanced anti-tumor efficiency with minimal side effects.

尽管越来越多的研究和纳米酶和氧化应激抗肿瘤疗法方面的最新进展，但是在正常组织中能够作为天然抗氧化系统维持氧化还原平衡的纳米颗粒仍然匮乏。在此，通过简单的水热法合成了磷酸铁（FePOs）基纳米酶。我们首先发现FePOs纳米酶具有类似三酶的活性。它在酸性肿瘤微环境中类似于过氧化物酶（POD），并且可以通过催化H ₂ O ₂生成·OH来介导高效的FePOs + H ₂ O ₂抗肿瘤方案。。与任何其他报道的纳米酶完全不同的是，在生理pH值下，FePOs不仅像过氧化氢酶（CAT）一样，而且像超氧化物歧化酶（SOD）一样。它对正常组织具有综合的SOD-CAT效应，类似于天然SOD-CAT对活生物体的协同效应，因此可以保护正常组织免受FePOs + H ₂ O ₂方案的伤害。发现细胞凋亡是诱导细胞死亡的途径。值得注意的是，FePOs + H ₂ O ₂由于联合的SOD-CAT效应，该方案未导致任何异常行为，明显的体重减轻或对小鼠正常组织的明显不利影响。所有这些有趣的结果证实，FePOs纳米酶是纳米药物的优秀候选者，并有望以最小的副作用提高抗肿瘤效率。