肥大细胞的新型活化和脱粒。,Biomedicine & Pharmacotherapy

当前位置： X-MOL 学术 › Biomed. Pharmacother. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

肥大细胞的新型活化和脱粒。
Biomedicine & Pharmacotherapy ( IF 6.9 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.biopha.2020.110157
Huanjin Liao ₁ , Xia Peng ₁ , Yiqin Ge ₁ , Yuting Liang ₁ , Yue Yin ₁ , Jia Li ₁ , Lihui Lin ₁ , Juan Wang ₁ , Yanning Li ₁ , Li Li ₁

Affiliation

肥大细胞（MCs）脱粒是过敏性炎症反应过程中的关键过程。MC激活后释放其预制的和新的合成颗粒。我们发现，在骨髓衍生的小鼠肥大细胞（BMMCs）激活后，颗粒被部分和选择性释放。接下来，我们研究了脱粒MC对新挑战的反应。BMMC被抗体/抗原（IgE / Ag）或化合物48/80（C48 / 80）激活。然后通过IgE / Ag或C48 / 80将脱粒的BMMC重新活化，而没有时间间隔。流式细胞术用于检测BMMCs的CD117，FcεRI和细胞内颗粒的表达，并使用β-己糖胺酶释放法检测BMMC的脱颗粒。通过用甲苯胺蓝染色观察BMMC的形态。由IgE / Ag激活的脱粒BMMC无法响应相同的IgE / Ag攻击，并释放β-己糖胺酶，而与未占用的FcεRI无关，但对C48 / 80产生了响应。由C48 / 80激活的脱粒BMMC对IgE / Ag或C48 / 80都有反应。这些结果表明，脱粒的BMMCs可以再次活化并释放颗粒介质，这揭示了脱粒的MCs独特的介质释放机制及其在维持炎症或引起超敏反应中的潜在功能。

"点击查看英文标题和摘要"

Novel reactivation and degranulation of mast cells.

Mast cells (MCs) degranulation is a key process during the allergic inflammatory response. MCs release their preformed and new synthesized granules after activation. We found that granules were released partially and selectively after the activation of bone marrow-derived mouse mast cells (BMMCs). Next, we investigated the response of degranulated MCs to a new challenge. BMMCs were activated by antibody/antigen (IgE/Ag) or compound 48/80 (C48/80). The degranulated BMMCs were then reactivated by either IgE/Ag or C48/80 without time intervals. Flow cytometry was used to detect the expression of CD117, FcεRI, and intracellular granules of BMMCs, and BMMCs degranulation was detected using the β-hexosaminidase release assay. The morphology of BMMCs was observed by staining with toluidine blue. Degranulated BMMCs activated by IgE/Ag failed to respond to the same IgE/Ag challenge and released β-hexosaminidase independent of unoccupied FcεRI, but responded to C48/80. Degranulated BMMCs activated by C48/80 responded to either IgE/Ag or C48/80. These results indicated that degranulated BMMCs could be reactivated and released granule mediators again, this revealed the unique mediator releasing mechanism of degranulated MCs and their potential function in maintaining inflammation or causing hypersensitivity.

更新日期：2020-05-06

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南