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Novel Pathway for Chloramphenicol Catabolism in the Activated Sludge Bacterial Isolate Sphingobium sp. CAP-1.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-05-15 , DOI: 10.1021/acs.est.9b07324 Xiaodan Ma 1 , Bin Liang 2, 3 , Mengyuan Qi 1 , Hui Yun 3, 4 , Ke Shi 1 , Zhiling Li 1 , Yuanqiang Guo 5 , Peisheng Yan 1 , Shuang-Jiang Liu 6 , Aijie Wang 1, 2, 3
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-05-15 , DOI: 10.1021/acs.est.9b07324 Xiaodan Ma 1 , Bin Liang 2, 3 , Mengyuan Qi 1 , Hui Yun 3, 4 , Ke Shi 1 , Zhiling Li 1 , Yuanqiang Guo 5 , Peisheng Yan 1 , Shuang-Jiang Liu 6 , Aijie Wang 1, 2, 3
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The chlorinated nitroaromatic antibiotic chloramphenicol (CAP) is a refractory contaminant that is widely present in various environments. However, few CAP-mineralizing bacteria have been documented, and a complete CAP catabolism pathway has yet to be identified. In this study, the bacterial strain Sphingobium sp. CAP-1 was isolated from an activated sludge sample and was shown to be capable of aerobically subsisting on CAP as the sole carbon, nitrogen, and energy source while simultaneously and efficiently degrading CAP. p-Nitrobenzoic acid (PNBA), p-nitrobenzaldehyde (PNBD), protocatechuate (PCA), and the novel side chain C3-hydroxy-oxygenated product of CAP (O-CAP) were identified during CAP degradation. Strain CAP-1 was able to convert O-CAP to intermediate product PNBA. The putative functional genes associated with PNBA catabolism into the tricarboxylic acid cycle via PCA and floc formation were also identified by genome sequencing and comparative proteome analysis. A complete pathway for CAP catabolism was proposed. The discovery of a novel CAP oxidation/detoxification process and a complete pathway for CAP catabolism enriches the fundamental understanding of the bacterial catabolism of antibiotics, providing new insights into the microbial-mediated fate, transformation, and resistance risk of CAP in the environment. The molecular basis of CAP catabolism and floc formation in strain CAP-1 also offers theoretical guidance for the enhanced bioremediation of CAP-containing environments.
中文翻译：
活性污泥细菌分离物鞘氨醇中的氯霉素分解代谢的新途径。CAP-1。
氯化硝基芳香族抗生素氯霉素（CAP）是一种难治性污染物，广泛存在于各种环境中。然而，很少有CAP矿化细菌的记录，并且尚未确定完整的CAP分解代谢途径。在这项研究中，细菌菌株鞘氨醇单胞菌。CAP-1是从活性污泥样品中分离出来的，并被证明能够作为唯一的碳，氮和能源有氧地存在于CAP上，同时又能有效降解CAP。对硝基苯甲酸（PNBA），对硝基苯甲醛（PNBD），原儿茶酸酯（PCA）和新型侧链C 3在CAP降解过程中确定了CAP的羟基氧化产物（O-CAP）。应变CAP-1能够将O-CAP转换为中间产品PNBA。还通过基因组测序和比较蛋白质组分析鉴定了与PNBA通过PCA分解代谢进入三羧酸循环和絮凝相关的功能基因。提出了CAP分解代谢的完整途径。新颖的CAP氧化/解毒过程以及CAP分解代谢的完整途径的发现丰富了对细菌细菌分解代谢的基本了解，从而为微生物介导的CAP在环境中的命运，转化和抗药性提供了新见解。
更新日期：2020-05-15
中文翻译：
活性污泥细菌分离物鞘氨醇中的氯霉素分解代谢的新途径。CAP-1。
氯化硝基芳香族抗生素氯霉素（CAP）是一种难治性污染物，广泛存在于各种环境中。然而，很少有CAP矿化细菌的记录，并且尚未确定完整的CAP分解代谢途径。在这项研究中，细菌菌株鞘氨醇单胞菌。CAP-1是从活性污泥样品中分离出来的，并被证明能够作为唯一的碳，氮和能源有氧地存在于CAP上，同时又能有效降解CAP。对硝基苯甲酸（PNBA），对硝基苯甲醛（PNBD），原儿茶酸酯（PCA）和新型侧链C 3在CAP降解过程中确定了CAP的羟基氧化产物（O-CAP）。应变CAP-1能够将O-CAP转换为中间产品PNBA。还通过基因组测序和比较蛋白质组分析鉴定了与PNBA通过PCA分解代谢进入三羧酸循环和絮凝相关的功能基因。提出了CAP分解代谢的完整途径。新颖的CAP氧化/解毒过程以及CAP分解代谢的完整途径的发现丰富了对细菌细菌分解代谢的基本了解，从而为微生物介导的CAP在环境中的命运，转化和抗药性提供了新见解。
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