Abstract Ganoderic acids (GAs) are oxygenated lanostane-type triterpenoids from the traditional medicinal mushroom Ganoderma lucidum and of significant biological activities. Although a ganoderic acid 3-hydroxy-lanosta-8,24-dien-26-oic acid (HLDOA) was found to be biosynthesized from lanosterol, further post-modification of HLDOA is yet unclear. In this work, by using HLDOA as the substrate and a crude enzyme from G. lucidum as the biocatalyst, we observed a new peak in liquid chromatography from the reaction system. The product was purified and identified to be 3-oxo-lanosta-8,24-dien-26-oic acid (OLDOA), which may be converted from HLDOA by a putative dehydrogenase of G. lucidum. The work is useful to future manufacture of GAs as well as their biosynthetic pathway elucidation.

中文翻译：

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灵芝粗酶对灵芝酸 3-hydroxy-lanosta-8,24-dien-26-oic 酸的生物转化

摘要 灵芝酸（GAs）是来自传统药用蘑菇灵芝的氧化羊毛脂类三萜类化合物，具有显着的生物活性。尽管发现灵芝酸 3-hydroxy-lanosta-8,24-dien-26-oic acid (HLDOA) 是由羊毛甾醇生物合成的，但 HLDOA 的进一步后修饰尚不清楚。在这项工作中，通过使用HLDOA作为底物和灵芝粗酶作为生物催化剂，我们在反应体系的液相色谱中观察到一个新峰。产物被纯化并鉴定为 3-oxo-lanosta-8,24-dien-26-oic acid (OLDOA)，它可以通过灵芝的推定脱氢酶从 HLDOA 转化而来。这项工作对未来 GA 的制造及其生物合成途径的阐明很有用。