The preparation of hydrogel wound dressing materials with high antibacterial activities and good biosafety simultaneously can be relatively challenging. Aiming at solving this crucial problem, a multi-functional chitosan/carboxymethyl chitosan/silver nanoparticles (CTS/CMCTS/AgNPs) polyelectrolyte composite physical hydrogel is constructed by in situ photoreduction of silver ions with α-hydroxy of CMCTS and semi-dissolution acidification sol-gel transition (SD-A-SGT) methods using natural polymers with no chemical reductant involved. The XRD, TEM and IR characterizations reveal a highly uniform structure of the composite hydrogel with good mechanical strength. The composite hydrogel also exhibits good self-healing ability, and the self-healing mechanism of the composite hydrogel prepared by natural polymers with high similarity is put forward on the basis of molecular dynamics simulation. The AgNPs gives the hydrogel excellent antibacterial activities against both S. aureus and P. aeruginosa., while the preparation method with no chemical reductant and crosslinking agent required endows the hydrogel high biocompatibility. The great application potentials of CTS/CMCTS/AgNPs polyelectrolyte composite hydrogel are demonstrated with its promoting effects on the healing of P. aeruginosa infected wounds as the wound dressing. It is a breakthrough in achieving hydrogels with desired biosafety and antibacterial activities simultaneously. The composite hydrogel is found with great application potentials as wound dressing.

同时制备具有高抗菌活性和良好生物安全性的水凝胶伤口敷料可能是相对具有挑战性的。为了解决这一关键问题，通过原位构建多功能壳聚糖/羧甲基壳聚糖/银纳米粒子（CTS / CMCTS / AgNPs）聚电解质复合物理水凝胶使用不包含化学还原剂的天然聚合物，通过CMCTS的α-羟基对银离子进行光还原和半溶解酸化溶胶-凝胶转变（SD-A-SGT）方法。XRD，TEM和IR表征表明，复合水凝胶具有高度均匀的结构，并具有良好的机械强度。该复合水凝胶还表现出良好的自愈能力，在分子动力学模拟的基础上，提出了由具有高相似性的天然聚合物制备的复合水凝胶的自愈机理。AgNPs使水凝胶对金黄色葡萄球菌和铜绿假单胞菌均具有出色的抗菌活性。，尽管不需要化学还原剂和交联剂的制备方法赋予水凝胶高的生物相容性。CTS / CMCTS / AgNPs聚电解质复合水凝胶作为伤口敷料具有促进铜绿假单胞菌感染伤口愈合的促进作用，具有巨大的应用潜力。这是在同时获得具有所需生物安全性和抗菌活性的水凝胶方面的突破。发现该复合水凝胶具有作为伤口敷料的巨大应用潜力。