2-Keto-d-gluconic acid (2KGA) is an important organic acid derived from d-glucose and is used to produce the food antioxidant erythorbic acid. To improve the 2KGA production performance and cell reusability, various carriers such as calcium alginate, k-carrageenan, chitosan, and poly(vinyl alcohol)-alginate were evaluated to immobilize Pseudomonas plecoglossicida JUIM01 resting cells. Calcium alginate was shown to be a suitable carrier since the immobilized cells had the highest number of reuse times and produced the highest 2KGA concentration of 171.77 g/L, with a productivity of 3.58 g/L·h and conversion ratio of 98.38%. The cell concentration, cultivation temperature, aeration rate and initial glucose concentration were further optimized in a 5-L airlift bioreactor to obtain the best 2KGA production performance by calcium alginate-immobilized P. plecoglossicida cells. Under the optimal conditions including a cell concentration of 4.0 g/L, glucose concentration of 126.0 g/L, temperature of 34 °C and aeration rate of 2.8 L/min, 134.45 g/L 2KGA was produced by alginate-immobilized P. plecoglossicida cells within 30 h, with a total productivity of 4.48 g/L·h and yield of 1.07 g/g (conversion ratio of over 99.0%). The immobilized cells maintained a stable conversion capacity after nine reuses and 25 days of storage at 4 °C, which indicated that calcium alginate immobilization of P. plecoglossicida cells had industrial practicability for 2KGA production.

中文翻译：

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固定的Pseudomonas plecoglossicida静息细胞从葡萄糖产生2-酮-葡萄糖酸。

2-酮-d-葡萄糖酸（2KGA）是一种衍生自d-葡萄糖的重要有机酸，用于生产食品抗氧化剂异抗坏血酸。为了提高2KGA的生产性能和细胞可重复使用性，评估了各种载体，例如海藻酸钙，k-角叉菜胶，壳聚糖和聚（乙烯醇）-海藻酸盐，以固定Pseudomonas plecoglossicida JUIM01静止细胞。海藻酸钙被证明是合适的载体，因为固定化的细胞具有最高的重复使用次数，并产生最高的2KGA浓度为171.77 g / L，产率为3.58 g / L·h，转化率为98.38％。在5-L气举生物反应器中进一步优化了细胞浓度，培养温度，通气速率和初始葡萄糖浓度，以通过藻酸钙固定化P获得最佳2KGA生产性能。plecoglossicida细胞。在最佳条件下，通过藻酸盐固定的P. plecoglossicida产生了134.45 g / L的2KGA，在细胞浓度为4.0 g / L，葡萄糖浓度为126.0 g / L，温度为34°C和通气率为2.8 L / min的条件下。 30 h内即可完成生产，总产量为4.48 g / L·h，产量为1.07 g / g（转化率超过99.0％）。固定的细胞在9次重复使用和在4°C下储存25天后保持了稳定的转化能力，这表明藻酸钙固定化plecoglossicida细胞具有工业实用性，可用于2KGA的生产。30 h内获得plecoglossicida细胞，总产量为4.48 g / L·h，产量为1.07 g / g（转化率超过99.0％）。固定的细胞在9次重复使用和在4°C下储存25天后保持了稳定的转化能力，这表明藻酸钙固定化plecoglossicida细胞具有工业实用性，可用于2KGA的生产。30 h内获得plecoglossicida细胞，总产量为4.48 g / L·h，产量为1.07 g / g（转化率超过99.0％）。固定的细胞在9次重复使用和在4°C下储存25天后保持了稳定的转化能力，这表明藻酸钙固定化P. plecoglossicida细胞具有工业实用性，可用于生产2KGA。