三肽Val-Pro-Pro（VPP）和Ile-Pro-Pro（IPP）通过干扰Ang II诱导的人脐静脉内皮细胞介导的EV将RNA传递至VSMC，从而调节血管平滑肌细胞的增殖和迁移模型。,Journal of Agricultural and Food Chemistry

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三肽Val-Pro-Pro（VPP）和Ile-Pro-Pro（IPP）通过干扰Ang II诱导的人脐静脉内皮细胞介导的EV将RNA传递至VSMC，从而调节血管平滑肌细胞的增殖和迁移模型。
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-05-14 , DOI: 10.1021/acs.jafc.0c02060
Tianyuan Song _{1,

2} , Miao Lv _{1,

2} , Baoguo Sun ₃ , Lin Zheng _{1,

2} , Mouming Zhao _{1,

2,

3}

Affiliation

血管紧张素II（Ang II）是肾素-血管紧张素-醛固酮系统（RAAS）的血管活性因子，可调节血管收缩并促进多种血管疾病。在这项研究中，有效的降压肽Val-Pro-Pro（VPP）和Ile-Pro-Pro（IPP）对来自血管内皮细胞的细胞外囊泡（EV）增殖和迁移血管平滑肌细胞（VSMC）的影响（VEC）使用细胞共培养模型进行了研究。分离，表征和研究了VEC衍生的EV。本研究表明，Ang II诱导的VEC产生的EV可以促进VSMCs的增殖，迁移和炎症因子（IL-6升高至40.75±4.33 pg / mL，IL-1β升高至28.62±5.42 pg / mL）。，VPP和IPP对此路径发挥了不同的抑制作用。带有RNase处理的电动汽车失去了对VSMC的作用，表明包装在囊泡中的RNA可能是关键的组成部分。这些结果表明，VPP和IPP可以通过调节EV介导的VEC和VSMC之间RNA的传递来减轻Ang II诱导的血管功能障碍。

"点击查看英文标题和摘要"

Tripeptides Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) Regulate the Proliferation and Migration of Vascular Smooth Muscle Cells by Interfering Ang II-Induced Human Umbilical Vein Endothelial Cells Derived EVs Delivering RNAs to VSMCs in the Co-culture Model.

Angiotensin II (Ang II), a vasoactive factor in the renin–angiotensin–aldosterone system (RAAS), can regulate vasoconstriction and promote multiple vascular diseases. In this study, the effects of potent antihypertensive peptide Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) on the proliferation and migration of vascular smooth muscle cells (VSMCs) by extracellular vesicles (EVs) from vascular endothelial cells (VECs) were studied using a cell co-culture model. The VEC-derived EVs were isolated, characterized, and investigated. The present study demonstrated that the EVs from Ang II-induced VECs could promote proliferation, migration, and inflammatory factors (IL-6 increased to 40.75 ± 4.33 pg/mL and IL-1β increased to 28.62 ± 5.42 pg/mL) generation of VSMCs, VPP and IPP exerted discrepant inhibitory effects on this pathway. The EVs with RNase treatment lost the effects on VSMCs, indicating that the RNAs packed into vesicles may be a critical component. These results implied that VPP and IPP could alleviate Ang II-induced vascular dysfunction by modulating the EV-mediated transmission of RNAs between VECs and VSMCs.

更新日期：2020-05-14

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