DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers.,Analyst

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DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers.
Analyst ( IF 3.6 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0an00656d
Yu-Hong Cheng ₁ , Hao Tang ₁ , Ru-Qin Yu ₁ , Jian-Hui Jiang ₁

Affiliation

We report a novel DNA-programmed plasmonic enzyme-linked immunosorbent assay (ELISA) for the ultrasensitive detection of protein biomarkers with the naked eye. The DNA-programmed plasmonic assay was based on two enzyme-free and isothermal nucleic acid amplification methods: hybridization chain reaction (HCR) and catalyzed hairpin assembly (CHA). In this study, a biotin-labeled DNA probe was utilized insteand of an enzyme-label probe in well-developed ELISA method. The biotin-labeled DNA probe was able to trigger the HCR and CHA processes, and the products could hybridize with DNA-modified gold nanoparticles (AuNPs) to induce the aggregation of the AuNPs and a color change in the solution. The developed method was able to detect as low as 1 pg mL⁻¹ PSA target with the naked eye. Clinical serum samples demonstrated satisfactory results, indicating that the method is useful for early diagnostics and monitoring curative effects after a medical treatment. The developed method presents a simple and portable platform for ultrasensitive protein detection and has potential for point-of-care (POC) diagnostics in less developed areas.

中文翻译：

DNA程序等离子体ELISA用于蛋白质生物标记物的超灵敏检测。

我们报告了一种新型的DNA程序化的等离激元酶联免疫吸附测定（ELISA），用肉眼可以超灵敏地检测蛋白质生物标志物。DNA编程的等离激元测定法基于两种无酶和等温核酸扩增方法：杂交链反应（HCR）和催化发夹装配（CHA）。在这项研究中，在发达的ELISA方法中，将生物素标记的DNA探针用于酶标记探针。生物素标记的DNA探针能够触发HCR和CHA过程，并且产物可以与DNA修饰的金纳米颗粒（AuNPs）杂交，以诱导AuNPs的聚集和溶液中的颜色变化。开发的方法能够检测低至1 pg mL ^-1用肉眼观察PSA目标。临床血清样本显示令人满意的结果，表明该方法可用于药物治疗后的早期诊断和监测疗效。所开发的方法提供了一种用于超灵敏蛋白质检测的简单且可移植的平台，并在欠发达地区具有即时诊断（POC）的潜力。

更新日期：2020-07-13

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