Identification and Mapping of Stable QTLs for Seed Oil and Protein Content in Soybean [Glycine max (L.) Merr.].,Journal of Agricultural and Food Chemistry

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Identification and Mapping of Stable QTLs for Seed Oil and Protein Content in Soybean [Glycine max (L.) Merr.].
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-05-13 , DOI: 10.1021/acs.jafc.0c01271
Jinghua Huang _{1,

2,

3} , Qibin Ma _{1,

2,

3} , Zhandong Cai _{1,

2,

3} , Qiuju Xia ₄ , Shuxian Li ₅ , Jia Jia _{1,

2,

3} , Li Chu _{1,

2,

3} , Tengxiang Lian _{1,

2,

3} , Hai Nian _{1,

2,

3} , Yanbo Cheng _{1,

2,

3}

Affiliation

This research aimed to identify stable quantitative trait loci (QTL) associated with oil and protein content in soybean. A population of 196 recombinant inbred lines (RILs) derived from Huachun 2 × Wayao was used to evaluate these target traits. A high-density genetic linkage map was constructed by using high-throughput genome-wide sequencing technology, which contained 3413 recombination bin markers and spanned 5400.4 cM with an average distance of 1.58 cM between markers. Eighteen stable QTLs controlling oil and protein content were detected. Among them, qOil-11-1 was identified for the first time as a novel QTL, while qOil-5-1, qPro-10-1, and qPro-14-1 were strong and stable QTLs with high log-likelihood (LOD) values. Sixteen differentially expressed genes (DEGs) within these four QTLs were shown to be highly expressed during seed development based on RNA sequencing (RNA-seq) data analysis. Our results may contribute toward gene mining and marker-assisted selection (MAS) for breeding a high-quality soybean in the future.

中文翻译：

大豆种子油和蛋白质含量稳定 QTL 的鉴定和定位 [Glycine max (L.) Merr.]。

本研究旨在鉴定与大豆油和蛋白质含量相关的稳定数量性状基因座（QTL）。使用源自华春 2 × 瓦窑的 196 个重组自交系 (RIL) 群体来评估这些目标性状。采用高通量全基因组测序技术构建高密度遗传连锁图谱，包含3413个重组箱标记，跨度5400.4cM，标记间平均距离1.58cM。检测到18个控制油脂和蛋白质含量的稳定QTL。其中， qOil-11-1首次被鉴定为新QTL，而qOil-5-1 、 qPro-10-1和qPro-14-1是强且稳定的高对数似然（LOD）QTL。）值。根据 RNA 测序 (RNA-seq) 数据分析，这 4 个 QTL 中的 16 个差异表达基因 (DEG) 在种子发育过程中高度表达。我们的结果可能有助于基因挖掘和标记辅助选择（MAS），以在未来培育优质大豆。

更新日期：2020-05-13

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