Application of Derivatization in Fatty Acids and Fatty Acyls Detection: Mass Spectrometry‐Based Targeted Lipidomics,Small Methods

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Application of Derivatization in Fatty Acids and Fatty Acyls Detection: Mass Spectrometry‐Based Targeted Lipidomics
Small Methods ( IF 10.7 ) Pub Date : 2020-05-11 , DOI: 10.1002/smtd.202000160
Li Xiang _{1,

2} , Lin Zhu ₁ , Yu Huang ₂ , Zongwei Cai ₁

Affiliation

Mass spectrometry (MS) has become an indispensable technique in the analysis of fatty acids (FAs) and fatty acyls due to its superior sensitivity and selectivity. Chemical derivatization is often introduced in MS‐based analysis because of the inherent poor ionization efficiency and selectivity of FAs and fatty acyls. This critical review presents an overview of commonly used MS platforms including gas chromatography‐MS, liquid chromatography‐MS, and mass spectrometry imaging combined with representative derivatization reagents for FAs and fatty acyls based on different chain‐lengths, saturation degrees, and conjugation groups. The method applications and limitations as well as critical derivatization conditions in different biological matrices are summarized. Special remarks on the chemical derivatization for MS‐based analysis are provided. Future perspectives associated with the detection of FAs and fatty acyls are discussed. This review provides valuable information and suggestions for the targeted detection of interested FAs and fatty acyls using different MS‐based platforms.

中文翻译：

衍生化在脂肪酸和脂肪酰基检测中的应用：基于质谱的靶向脂质组学

质谱（MS）由于其优越的灵敏度和选择性，已成为分析脂肪酸（FAs）和脂肪酰基的必不可少的技术。由于固有的弱电离效率以及FA和脂肪酰的选择性，MS的分析中经常引入化学衍生化。这篇重要的评论文章概述了常用的MS平台，包括基于气相色谱-质谱，液相色谱-MS和质谱成像以及基于不同链长，饱和度和结合基团的FAs和脂肪酰基的代表性衍生化试剂。总结了该方法在各种生物基质中的应用和局限性以及关键的衍生条件。提供了有关基于MS的分析的化学衍生化的特别说明。讨论了与FAs和脂肪酰基检测有关的未来观点。这篇综述为使用不同的基于MS的平台针对感兴趣的FA和脂肪酰基进行有针对性的检测提供了有价值的信息和建议。

更新日期：2020-05-11

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