Abstract In both chemo- and biocatalysis the immobilization of catalysts to carriers is often beneficial in terms of catalytic activity and ease of operation. In the present study we encapsulated an enzyme, β-D-glucosidase, inside thermosensitive poly(N-acryloyl glycinamide) microgels by radical polymerization of N-acryloyl glycinamide in the presence of the enzymes. Properties of these hybrid microgels were studied varying the enzyme-monomer ratio and the degree of crosslinking. The enzymatic activities of the microgels were assessed using a model reaction, enzymatic cleavage of p-nitrophenyl-β-D-glucopyranoside under different conditions. The microgel encapsulated enzymes showed enhanced activity at high pH compared to the native enzymes. Once the enzymatic activity of the microgels was ascertained, introduction of silver nanoparticles inside the enzyme carrying microgels was made to develop bicatalytic systems. The bicatalytic microgels were shown to be capable of carrying out a cascade reaction combining enzymatic catalysis and reduction of the reaction product 4-nitrophenol to 4-aminophenol.

中文翻译：

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双催化聚（N-丙烯酰甘氨酰胺）微凝胶

摘要 在化学和生物催化中，将催化剂固定在载体上通常有利于提高催化活性和易于操作。在本研究中，我们通过 N-丙烯酰甘氨酰胺在酶的存在下进行自由基聚合，将一种酶 β-D-葡萄糖苷酶封装在热敏聚（N-丙烯酰甘氨酰胺）微凝胶内。研究了这些混合微凝胶的特性，改变了酶-单体比例和交联度。使用模型反应评估微凝胶的酶促活性，在不同条件下对硝基苯基-β-D-吡喃葡萄糖苷进行酶促裂解。与天然酶相比，微凝胶封装的酶在高 pH 值下显示出增强的活性。一旦确定了微凝胶的酶活性，在携带酶的微凝胶中引入银纳米粒子以开发双催化系统。双催化微凝胶能够进行级联反应，将酶催化和反应产物 4-硝基苯酚还原为 4-氨基苯酚。