Anthocyanin Profiling of Maize Grains Using DIESI-MSQD Reveals That Cyanidin-Based Derivatives Predominate in Purple Corn, whereas Pelargonidin-Based Molecules Occur in Red-Pink Varieties from Mexico.,Journal of Agricultural and Food Chemistry

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Anthocyanin Profiling of Maize Grains Using DIESI-MSQD Reveals That Cyanidin-Based Derivatives Predominate in Purple Corn, whereas Pelargonidin-Based Molecules Occur in Red-Pink Varieties from Mexico.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-05-07 , DOI: 10.1021/acs.jafc.9b06336
Héctor A Peniche-Pavía ₁ , Axel Tiessen _{1,

2}

Affiliation

Corn seeds contain natural pigments and antioxidants, such as the molecular variants of flavonoids and carotenoids. The aleurone and pericarp tissues from pigmented genotypes were extracted for metabolic fingerprinting and evaluated using UV-vis and mass spectrometry (MS). MS ionomic fingerprints classified samples according to genetic background and kernel color. The MS/MS fragmentation pattern (Daughter and Neutral Loss methods) allowed the tentative identification of 18 anthocyanins with glycosyl, malonyl, and succinyl moieties, including 535 m/z for cyanidin-3-O-(6″-malonyl-glucoside) and 621 m/z for cyanidin-3-O-(3″,6″-dimalonyl-glucoside). We also detected 663 m/z for pelargonidin-3-O-(disuccinyl-glucoside) and 633 m/z for peonidin-3-O-(disuccinyl-glucoside). Cyanidin-based anthocyanins were the most abundant in dark purple colored kernels, while pelargonidins predominated in the red-pink kernels of the "Elote occidental" landrace. Grains of "Conico negro" had a simultaneous pigmentation of aleurone and pericarp, while Vitamaize had purple pigmentation only in the aleurone layer. Most landraces had a white endosperm, while Vitamaize had a yellow endosperm and a dark seed coat. We conclude that Vitamaize grains contain both carotenes and anthocyanins, and therefore it is proposed as a nontransgenic agronomically improved variety of tropical purple maize, a good source for organic superfoods.

中文翻译：

使用DIESI-MSQD对玉米籽粒进行花色苷分析的研究表明，基于玉米醇溶蛋白的衍生物在紫色玉米中占主导地位，而基于花生四烯酸的分子在墨西哥的红粉红色品种中发生。

玉米种子包含天然色素和抗氧化剂，例如类黄酮和类胡萝卜素的分子变体。提取有色基因型的糊粉和果皮组织进行代谢指纹分析，并使用紫外可见和质谱（MS）进行评估。MS ionomic指纹图谱根据遗传背景和籽粒颜色对样品进行分类。MS / MS碎片模式（Daughter和Neutral Loss方法）允许初步鉴定18个具有糖基，丙二酰基和琥珀酰基部分的花色苷，其中cyanidin-3-O-（6″-丙二酰基-葡糖苷）的535 m / z对于花青素-3-O-（3″，6″-二烯丙基-葡萄糖苷）为621m / z。我们还检测到pelargonidin-3-O-（二琥珀酰葡萄糖苷）为663 m / z和peonidin-3-O-（二琥珀酰葡萄糖苷）为633 m / z。氰基花青素含量最高，为深紫色粒，而pelargonidins在“ Elote西方”地方品种的红粉红色内核中占主导地位。“ Conico negro”的谷物同时具有糊粉糊和果皮的色素沉着，而Vitamaize仅在糊粉糊层中具有紫色的色素沉着。大多数地方品种的胚乳是白色的，而Vitamaize的胚乳是黄色的，种皮是深色的。我们得出的结论是，Vitamaize谷物同时含有胡萝卜素和花色苷，因此被提议作为一种非转基因农艺改良品种，即热带紫色玉米，是有机超级食品的良好来源。大多数地方品种的胚乳是白色的，而Vitamaize的胚乳是黄色的，种皮是深色的。我们得出的结论是，Vitamaize谷物同时含有胡萝卜素和花色苷，因此被提议作为一种非转基因农艺改良品种，即热带紫色玉米，是有机超级食品的良好来源。大多数地方品种的胚乳是白色的，而Vitamaize的胚乳是黄色的，种皮是深色的。我们得出的结论是，Vitamaize谷物同时含有胡萝卜素和花色苷，因此被提议作为一种非转基因农艺改良品种，即热带紫色玉米，是有机超级食品的良好来源。

更新日期：2020-05-07

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