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Comparative proteomics reveals stress responses of Vibrio parahaemolyticus biofilm on different surfaces: Internal adaptation and external adjustment.
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.scitotenv.2020.138386 Linxia Guo 1 , Jingjing Wang 2 , Yi Gou 1 , Ling Tan 1 , Haiquan Liu 3 , Yingjie Pan 4 , Yong Zhao 4
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-05-06 , DOI: 10.1016/j.scitotenv.2020.138386 Linxia Guo 1 , Jingjing Wang 2 , Yi Gou 1 , Ling Tan 1 , Haiquan Liu 3 , Yingjie Pan 4 , Yong Zhao 4
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Vibrio parahaemolyticus is a kind of gram-negative marine pathogen, which usually adheres to stainless steel (SS), glass (GS) and other abiotic surfaces in aquaculture and food processing in the form of biofilm and causes the spread of gastrointestinal illness. However, the deeply survival adaptation mechanism of V. parahaemolyticus biofilm cells on these contact surface remained unclear. Here, proteomics was used to investigated the physiological response of the V. parahaemolyticus biofilms cells to different abiotic surfaces (SS, GS and polystyrene (PS)). In addition, the effect of contact materials on the physical-chemical properties of biofilms are also characterized. Results showed that the expression of proteins of biofilm cells established on the SS surface were mainly related to the alleviation of metal ion stress and toxicity. The up-regulated proteins in the biofilm cells formed on the GS surface were mainly involved in the biological processes of sugar uptake, protein synthesis and bacterial chemotaxis. Meanwhile, the significantly expressed proteins in the biofilm cells formed on the PS surface were mainly involved in the cellular physiological activity of aromatic compound metabolism, osmotic stress and nutrient transport. All functional proteins mentioned above were closely related to the interaction characteristics of the contact surface and biofilm. This study provided an in-depth comparison of V. parahaemolyticus biofilm formation on these three abiotic surfaces, and presented a model in first time for the adaptation behavior of biofilm cells on different surfaces as affected by metal ion stress, nutrition, osmotic stress, and sugar utilization, which could facilitate an efficient control strategy for biofilm formation in industrial field.
中文翻译：
比较蛋白质组学揭示了副溶血性弧菌生物膜在不同表面上的应激反应：内部适应和外部调节。
副溶血性弧菌是一种革兰氏阴性海洋病原体，通常以生物膜的形式粘附在水产养殖和食品加工中的不锈钢（SS），玻璃（GS）和其他非生物表面上，并引起胃肠道疾病的传播。但是，尚不清楚这些接触表面上副溶血性弧菌生物膜细胞的深度存活适应机制。在这里，蛋白质组学被用来研究副溶血弧菌生物膜细胞对不同非生物表面（SS，GS和聚苯乙烯（PS））的生理反应。另外，还表征了接触材料对生物膜的物理化学性质的影响。结果表明，在SS表面上建立的生物膜细胞的蛋白质表达主要与减轻金属离子胁迫和毒性有关。GS表面上形成的生物膜细胞中的上调蛋白质主要参与糖吸收，蛋白质合成和细菌趋化性的生物学过程。同时，在PS表面形成的生物膜细胞中大量表达的蛋白质主要参与芳香族化合物代谢，渗透压和营养物质转运的细胞生理活性。上面提到的所有功能蛋白都与接触表面和生物膜的相互作用特性密切相关。这项研究提供了在这三个非生物表面上副溶血性弧菌生物膜形成的深入比较，并首次提出了一个模型，该模型针对生物膜细胞在不同表面上受金属离子应力，营养，渗透压和氧化应激影响的适应行为。糖利用
更新日期：2020-05-06
中文翻译：
比较蛋白质组学揭示了副溶血性弧菌生物膜在不同表面上的应激反应：内部适应和外部调节。
副溶血性弧菌是一种革兰氏阴性海洋病原体，通常以生物膜的形式粘附在水产养殖和食品加工中的不锈钢（SS），玻璃（GS）和其他非生物表面上，并引起胃肠道疾病的传播。但是，尚不清楚这些接触表面上副溶血性弧菌生物膜细胞的深度存活适应机制。在这里，蛋白质组学被用来研究副溶血弧菌生物膜细胞对不同非生物表面（SS，GS和聚苯乙烯（PS））的生理反应。另外，还表征了接触材料对生物膜的物理化学性质的影响。结果表明，在SS表面上建立的生物膜细胞的蛋白质表达主要与减轻金属离子胁迫和毒性有关。GS表面上形成的生物膜细胞中的上调蛋白质主要参与糖吸收，蛋白质合成和细菌趋化性的生物学过程。同时，在PS表面形成的生物膜细胞中大量表达的蛋白质主要参与芳香族化合物代谢，渗透压和营养物质转运的细胞生理活性。上面提到的所有功能蛋白都与接触表面和生物膜的相互作用特性密切相关。这项研究提供了在这三个非生物表面上副溶血性弧菌生物膜形成的深入比较，并首次提出了一个模型，该模型针对生物膜细胞在不同表面上受金属离子应力，营养，渗透压和氧化应激影响的适应行为。糖利用
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