Enhanced performance of an electrochemical aptasensor for real-time detection of vascular endothelial growth factor (VEGF) by nanofabrication and ratiometric measurement,Analytica Chimica Acta

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Enhanced performance of an electrochemical aptasensor for real-time detection of vascular endothelial growth factor (VEGF) by nanofabrication and ratiometric measurement
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.aca.2020.05.003
Shengnan Ni ₁ , Zhuping Shen ₁ , Pengfei Zhang ₂ , Guozhen Liu ₃

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Achieving a biosensing interface without baseline drift caused by variables in matrix samples is essential for real-time detection of analytes. In this study, we developed a molecular beacon based electrochemical aptasensor to realize the ratiometric signal quantification of VEGF in serum by surface modification of nanocomposites of graphene oxide/methylene blue (GO/MB) and AuNPs followed by the attachment of ferrocene-labeled aptamer (aptamer-Fc) against VEGF. The presence of VEGF can trigger the configuration change of aptamer-Fc, resulting in the redox probe Fc being far away from the electrode surface to attenuate the electrochemical communication between electrode and Fc. Meanwhile, signal of MB also decreased due to the impediment of aptamer-Fc to electron transfer passage. The achieved GC-rGO/MB-AuNPs-aptamer-Fc sensing interface was successfully used for the sensitive detection of VEGF in real-time with a linear detection range 2-500 pg mL-1 and detection limit of 0.1 pg mL-1 based on ratiometric dual signal (Fc and MB) read-out. It was observed loading MB and AuNPs to the GO based sensing interface was favorable to enhance the analytical performance in terms of sensitivity and capability to effectively eliminate background interference. This electrochemical aptasensor provides a universal and reliable biosensing platform which is potential for real-time and sensitive tracking of various cytokines in vivo.

中文翻译：

通过纳米加工和比率测量提高电化学适体传感器实时检测血管内皮生长因子 (VEGF) 的性能

实现没有由基质样品中的变量引起的基线漂移的生物传感界面对于分析物的实时检测至关重要。在这项研究中，我们开发了一种基于分子信标的电化学适体传感器，通过对氧化石墨烯/亚甲蓝 (GO/MB) 和 AuNPs 的纳米复合材料进行表面修饰，然后附着二茂铁标记的适体，实现血清中 VEGF 的比率信号量化。适配体-Fc) 抗 VEGF。VEGF的存在会触发适配体-Fc的构型变化，导致氧化还原探针Fc远离电极表面，减弱电极与Fc之间的电化学通讯。同时，由于适体-Fc 阻碍电子传递，MB 的信号也降低。获得的GC-rGO/MB-AuNPs-aptamer-Fc传感界面成功用于VEGF的实时灵敏检测，线性检测范围为2-500 pg mL-1，检测限为0.1 pg mL-1比率双信号（Fc 和 MB）读出。观察到将 MB 和 AuNPs 加载到基于 GO 的传感界面有利于提高灵敏度和有效消除背景干扰的能力方面的分析性能。这种电化学适体传感器提供了一个通用且可靠的生物传感平台，有可能在体内实时、灵敏地跟踪各种细胞因子。观察到将 MB 和 AuNPs 加载到基于 GO 的传感界面有利于提高灵敏度和有效消除背景干扰的能力方面的分析性能。这种电化学适体传感器提供了一个通用且可靠的生物传感平台，有可能在体内实时、灵敏地跟踪各种细胞因子。观察到将 MB 和 AuNPs 加载到基于 GO 的传感界面有利于提高灵敏度和有效消除背景干扰的能力方面的分析性能。这种电化学适体传感器提供了一个通用且可靠的生物传感平台，有可能在体内实时、灵敏地跟踪各种细胞因子。

更新日期：2020-07-01

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