The regulation of transcription level is an important step in gene expression process. Beauveria bassiana is a broad-spectrum insecticidal fungi widely used in the biologic control of arthropod. The regulation of its transcription level is a multilevel complex process. Multiprotein bridging factor 1(MBF1) is a transcriptional co-activator that bridges sequence-specific activators and the TATA-box binding protein(TBP), Little is known about the interaction between MBF1, TBP, and TBP binding to DNA(TATA-sequences)in filamentous fungi of Beauveria bassiana, The binding of TBP to TATA-box and TBP to MBF1 was investigated via electrochemical biosensor. Graphene oxide has an electronic mobility that is unattainable for any metal, so it will be highly sensitive as a test electrode. Hence, we developed a simple, sensitive and specific sensor based on an TBP probe and graphene oxide that successfully detected the interaction of TBP and TATA-box or MBF1. From the electrochemical impedance spectroscopy (EIS), we find that the radius will increase when adding TATA-box or MBF1 buffer to the modified TBP protein electrode. When adding no TATA-box or no MBF1, the radius is relatively unchanged. The interaction between TBP and TATA-box or MBF1 was proved based on the results. These data confirmed the specificity of the interactions, (1) our developed graphene-based electrochemical biosensor can be used for monitoring the interaction between TBP and TATA-box or MBF1, (2) TBP can bind to TATA-box, (3) TBP can bind to MBF1, and (4) TBP mediates the interactions of MBF1 to DNA. Therefore, this work provided a label-free, low-cost and simple detection method for the complex process of eukaryotic gene transcription regulation.

中文翻译：

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基于石墨烯的电化学生物传感器研究球孢白僵菌中TATA-Box结合蛋白（TBP），TATA-Box和多蛋白桥连因子1（MBF1）之间的相互作用。

转录水平的调控是基因表达过程中的重要步骤。球孢白僵菌是一种广谱杀虫真菌，广泛用于节肢动物的生物防治。其转录水平的调节是一个多层次的复杂过程。多蛋白桥连因子1（MBF1）是一种转录共激活因子，可桥接序列特异性激活因子和TATA-box结合蛋白（TBP），但对MBF1，TBP和TBP与DNA结合的相互作用（TATA序列）知之甚少在球孢白僵菌的丝状真菌中，通过电化学生物传感器研究了TBP与TATA-box的结合以及TBP与MBF1的结合。氧化石墨烯具有任何金属都无法实现的电子迁移率，因此作为测试电极将非常敏感。因此，我们开发了一个简单的 基于TBP探针和氧化石墨烯的灵敏而特异的传感器，可以成功检测到TBP与TATA-box或MBF1的相互作用。从电化学阻抗谱（EIS），我们发现当将TATA-box或MBF1缓冲液添加到修饰的TBP蛋白电极上时，半径将增加。当不添加TATA盒或MBF1时，半径相对不变。结果证明了TBP与TATA-box或MBF1之间的相互作用。这些数据证实了相互作用的特异性，（1）我们开发的基于石墨烯的电化学生物传感器可用于监测TBP与TATA-box或MBF1之间的相互作用，（2）TBP可与TATA-box结合，（3）TBP可以与MBF1结合，（4）TBP介导MBF1与DNA的相互作用。因此，这项工作提供了无标签，