Importance of protein Ser/Thr/Tyr phosphorylation for bacterial pathogenesis,FEBS Letters

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Importance of protein Ser/Thr/Tyr phosphorylation for bacterial pathogenesis
FEBS Letters ( IF 3.0 ) Pub Date : 2020-06-17 , DOI: 10.1002/1873-3468.13797
Julie Bonne Køhler ₁ , Carsten Jers ₁ , Mériem Senissar ₁ , Lei Shi ₂ , Abderahmane Derouiche ₂ , Ivan Mijakovic _{1,

2}

Affiliation

Protein phosphorylation regulates a large variety of biological processes in all living cells. In pathogenic bacteria, the study of serine, threonine and tyrosine (Ser/Thr/Tyr) phosphorylation has shed light on the course of infectious diseases, from adherence to host cells to pathogen virulence, replication and persistence. Mass spectrometry (MS)-based phosphoproteomics has provided global maps of Ser/Thr/Tyr phosphosites in bacterial pathogens. Despite recent developments, a quantitative and dynamic view of phosphorylation events that occur during bacterial pathogenesis is currently lacking. Temporal, spatial and sub-population resolution of phosphorylation data is required to identify key regulatory nodes underlying bacterial pathogenesis. Herein we discuss how technological improvements in sample handling, MS instrumentation, data processing and machine learning should improve bacterial phosphoproteomic datasets and the information extracted from them. Such information is expected to significantly extend the current knowledge of Ser/Thr/Tyr phosphorylation in pathogenic bacteria and should ultimately contribute to the design of novel strategies to combat bacterial infections.

中文翻译：

蛋白质 Ser/Thr/Tyr 磷酸化对细菌发病机制的重要性

蛋白质磷酸化调节所有活细胞中的多种生物过程。在病原菌中，对丝氨酸、苏氨酸和酪氨酸 (Ser/Thr/Tyr) 磷酸化的研究揭示了传染病的过程，从粘附到宿主细胞到病原体毒力、复制和持久性。基于质谱 (MS) 的磷酸化蛋白质组学提供了细菌病原体中 Ser/Thr/Tyr 磷酸位点的全局图谱。尽管最近有发展，但目前仍缺乏对细菌发病过程中发生的磷酸化事件的定量和动态视图。需要磷酸化数据的时间、空间和亚群分辨率来识别细菌发病机制的关键调控节点。在此我们讨论如何在样品处理、MS 仪器、数据处理和机器学习应该改进细菌磷酸蛋白质组数据集和从中提取的信息。预计这些信息将显着扩展目前对病原菌中 Ser/Thr/Tyr 磷酸化的了解，并最终有助于设计对抗细菌感染的新策略。

更新日期：2020-06-17

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