A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging.,Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

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A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-05-05 , DOI: 10.1016/j.saa.2020.118405
Defang Han ₁ , Jundan Yi ₁ , Chang Liu ₂ , Lijuan Liang ₃ , Kun Huang ₁ , Linhai Jing ₁ , Dabin Qin ₁

Affiliation

A new fluorescent probe Lyso-Fl has been facilely prepared by an esterification reaction of spironolactone fluoran dye Rdi with ethanol, which shows viscosity-selective response by fluorescence. The new probe delivers obvious fluorescence signal enhancement when environmental viscosity changes from 1.01 cP (water) to 1256 cP (98% glycerol). And, both the emission intensity (575 nm) and fluorescence lifetime of Lyso-Fl exhibit individually good linear relationships with the solution viscosity. Besides, Lyso-Fl gives a selective response to viscosity among various biological species and exhibits pH-independent (1-10) fluorescent signals towards viscosity. More importantly, Lyso-Fl shows low cytotoxicity and can be utilized for monitoring of dexamethasone-stimulated viscosity enhancement by cell imaging with excellent lysosome-targeted performance, promoting it a promising fluorescent probe for lysosomal viscosity detection.

中文翻译：

基于荧光素的粘度探针，用于靶向溶酶体的荧光成像。

通过螺内酯荧烷荧光染料Rdi与乙醇的酯化反应已经很容易地制备了新的荧光探针Lyso-F1，该荧光探针显示了荧光的粘度选择性反应。当环境粘度从1.01 cP（水）变为1256 cP（98％甘油）时，新探针可显着增强荧光信号。并且，Lyso-F1的发射强度（575nm）和荧光寿命均显示出与溶液粘度的良好线性关系。此外，Lyso-F1对多种生物物种之间的粘度产生选择性响应，并且对粘度表现出不依赖于pH的（1-10）荧光信号。更重要的是，Lyso-F1具有较低的细胞毒性，可用于通过细胞成像监测地塞米松刺激的粘度增强，并具有出色的靶向溶酶体的性能，

更新日期：2020-05-05

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