Prussian Blue nanoparticles (PBNPs) were utilized in a lateral flow immunoassay (LFA) and in an indirect competitive nanozyme-linked immunosorbent assay (icELISA), respectively, for their intense blue color and peroxidase (POx) -like activity. The PBNPs with good POx-like activity was linked to the antibody. Under the optimal parameters, both the PBNP-icELISA and PBNP-LFA perform very well. The icELISA has an IC50 value of 190 ng/mL, the working range extends from 29 to 1200 ng/mL, and the limit of detection is 22 ng/mL. The visual cut-off limit is 10 ng/mL. The dual immunoassay was used to quantify glycocholic acid in spiked human urine. Excellent recoveries and correlation between the two methods were observed.

中文翻译：

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具有双重过氧化物酶模拟特性的普鲁士蓝纳米颗粒，可用于糖胆酸的双重免疫分析。

普鲁士蓝纳米颗粒（PBNP）分别在侧向流免疫测定（LFA）和间接竞争性纳米酶联免疫吸附测定（icELISA）中被利用，因为它们具有强烈的蓝色和过氧化物酶（POx）样活性。具有良好POx样活性的PBNP与抗体连接。在最佳参数下，PBNP-icELISA和PBNP-LFA均表现出色。icELISA的IC50值为190 ng / mL，工作范围从29到1200 ng / mL，检测极限为22 ng / mL。视觉截止极限为10 ng / mL。双重免疫测定法用于定量加标的人类尿液中的糖胆酸。观察到两种方法之间的回收率和相关性极好。