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Near-Infrared Electrochemiluminescence Immunoassay with Biocompatible Au Nanoclusters as Tags.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-05-05 , DOI: 10.1021/acs.analchem.0c00125
Lei Yu 1, 2 , Qiao Zhang 1 , Qi Kang 1 , Bin Zhang 3 , Dazhong Shen 1 , Guizheng Zou 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-05-05 , DOI: 10.1021/acs.analchem.0c00125
Lei Yu 1, 2 , Qiao Zhang 1 , Qi Kang 1 , Bin Zhang 3 , Dazhong Shen 1 , Guizheng Zou 3
Affiliation
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The design and development of novel electrochemiluminescence (ECL) systems with near-infrared (NIR) emission beyond 800 nm are promising for ECL evolution, especially for improving the throughput of the spectrum-resolved multiplexing ECL assay and biological imaging. Herein a biocompatible and environmentally friendly luminophore, that is, the methionine-stabilized Au nanoclusters (Met-Au NCs), are proposed to achieve efficient aqueous ECL of ∼835 nm with triethanolamine as coreactant. The Met-Au NCs not only demonstrate a 75 times enhanced ECL compared with the traditional Au NCs with bovine serum albumin as a capping agent but also can be employed as ECL tags to label proteins with a methionine linker and enable a highly sensitive NIR ECL bioassay. A sandwich-type NIR ECL immunosensor is constructed with the Met-Au NCs as tags and α-fetoprotein (AFP) as a model analyte and exhibits a wide linearity range from 3 fg·mL–1 to 0.1 ng·mL–1 with a limit of detection of 1 fg mL–1 (S/N = 3) as well as desired selectivity.
中文翻译：
以生物相容性金纳米簇为标记的近红外电化学发光免疫分析。
具有超过800 nm的近红外（NIR）发射的新型电化学发光（ECL）系统的设计和开发对于ECL的发展是有希望的，特别是对于提高光谱分辨的多重ECL分析和生物成像的通量。在本文中，提出了一种生物相容且对环境友好的发光体，即蛋氨酸稳定的Au纳米团簇（Met-Au NCs），以三乙醇胺为共活化剂可实现约835 nm的有效水性ECL。与使用牛血清白蛋白作为封端剂的传统Au NCs相比，Met-Au NCs不仅显示出增强了75倍的ECL，而且还可以用作ECL标签，用蛋氨酸接头标记蛋白质并实现高度灵敏的NIR ECL生物测定。–1至0.1 ng·mL –1，检测限为1 fg mL –1（S / N = 3）以及所需的选择性。
更新日期：2020-05-05
中文翻译：
以生物相容性金纳米簇为标记的近红外电化学发光免疫分析。
具有超过800 nm的近红外（NIR）发射的新型电化学发光（ECL）系统的设计和开发对于ECL的发展是有希望的，特别是对于提高光谱分辨的多重ECL分析和生物成像的通量。在本文中，提出了一种生物相容且对环境友好的发光体，即蛋氨酸稳定的Au纳米团簇（Met-Au NCs），以三乙醇胺为共活化剂可实现约835 nm的有效水性ECL。与使用牛血清白蛋白作为封端剂的传统Au NCs相比，Met-Au NCs不仅显示出增强了75倍的ECL，而且还可以用作ECL标签，用蛋氨酸接头标记蛋白质并实现高度灵敏的NIR ECL生物测定。–1至0.1 ng·mL –1，检测限为1 fg mL –1（S / N = 3）以及所需的选择性。
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