Electrochemical Cloth-Based DNA Sensors (ECDSs): A New Class of Electrochemical Gene Sensors.,Analytical Chemistry

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Electrochemical Cloth-Based DNA Sensors (ECDSs): A New Class of Electrochemical Gene Sensors.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-05-05 , DOI: 10.1021/acs.analchem.0c00669
Jun Jiang _{1,

2} , Hongyang Wu _{1,

2} , Yan Su _{1,

2} , Yi Liang _{1,

2} , Bowen Shu ₃ , Chunsun Zhang _{1,

2}

Affiliation

Electrochemical (EC) sensors have been widely developed for DNA detection, but they are seldom used in a simple, economic, and efficient manner. In this work, for the first time, EC cloth-based DNA sensors (ECDSs) are developed as a new class of EC DNA sensors, without the need for cumbersome chip fabrication and high-cost peripheral facilities. Carbon ink- and solid wax-based screen printing were used to produce ultracheap sensing devices (the cost of one sensor is estimated to be $0.045). Also, a CdTe QDs/MWCNTs nanocomposite (CdTe-MWCNTs) was applied to modify the sensing interface to obtain a stronger EC signal. Specifically, the newly developed double linear hybridization chain reaction (DL-HCR) greatly amplified the EC signal, relative to the conventional linear HCR. Under optimized conditions, target DNA (TD) samples (75-bp DNA fragments prepared via PCR amplification) were determined in a range from 20 fM to 5 nM, with a detection limit of 8.74 fM and relative standard deviations of 2.04% and 4.75% for intra- and inter-assays at 50 pM TD, respectively. Additionally, the ECDSs had an acceptable storage stability and high selectivity. Importantly, the ECDSs, coupled with simple enzyme digestion, could detect genomic DNA from Listeria monocytogenes (L. monocytogenes), and a detection limit of 0.039 ng/μL was obtained. When coupled with enzyme digestion and PCR amplification, the ECDSs could determine L. monocytogenes in milk samples, with detection limits of approximately 1.64 × 10⁴ and 11 CFU/mL. These results demonstrate that the method offers a broad prospect for cost-effective, reliable, and highly sensitive gene-sensing applications.

中文翻译：

基于电化学布料的DNA传感器（ECDS）：新型的电化学基因传感器。

电化学（EC）传感器已广泛用于DNA检测，但很少以简单，经济和有效的方式使用。在这项工作中，基于EC布的DNA传感器（ECDS）首次被开发为新型的EC DNA传感器，而无需繁琐的芯片制造和昂贵的外围设备。使用基于碳墨水和固体蜡的丝网印刷来生产超便宜的传感设备（一个传感器的成本估计为0.045美元）。同样，使用CdTe QDs / MWCNTs纳米复合材料（CdTe-MWCNTs）修改感测界面以获得更强的EC信号。具体而言，相对于传统的线性HCR，新开发的双线性杂交链反应（DL-HCR）大大放大了EC信号。在优化条件下，测定目标DNA（TD）样品（通过PCR扩增制备的75 bp DNA片段）的范围为20 fM至5 nM，检出限为8.74 fM，内部和内部的相对标准偏差为2.04％和4.75％。分别以50 pM TD进行内部分析。另外，ECDS具有可接受的储存稳定性和高选择性。重要的是，ECDS与简单的酶消化结合，可以从单核细胞增生李斯特菌（L. monocytogenes），检出限为0.039 ng /μL。与酶消化和PCR扩增结合使用时，ECDS可以测定牛奶样品中的单核细胞增生李斯特菌，检出限约为1.64×10 ⁴和11 CFU / mL。这些结果表明，该方法为具有成本效益，可靠和高度敏感的基因传感应用提供了广阔的前景。

更新日期：2020-05-05

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