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Controlling the DNA Hybridization Chain Reaction
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-05-01 , DOI: 10.1021/jacs.0c02892 C Adrian Figg 1 , Peter H Winegar 1 , Oliver G Hayes 1 , Chad A Mirkin 1
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-05-01 , DOI: 10.1021/jacs.0c02892 C Adrian Figg 1 , Peter H Winegar 1 , Oliver G Hayes 1 , Chad A Mirkin 1
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A novel method for controlling the oligomerization of metastable DNA hairpins using the hybridization chain reaction (HCR) is reported. Control was achieved through the introduction of a base-pair mismatch in the duplex of the hairpins. The mismatch modification allows one to kinetically differentiate initiation versus propagation events, leading to DNA oligomers up to 10 monomers long and improving dispersities from 2.5 to 1.3-1.6. Importantly, even after two consecutive chain extensions, dispersity remained unaffected, showing that well-defined block co-oligomers can be achieved. As a proof-of-concept, this technique was then applied to hairpin monomers functionalized with a mutant green fluorescent protein to prepare protein oligomers. Taken together, this work introduces an effective method for controlling living macromolecular HCR oligomerization in a manner analogous to the controlled polymerization of small molecules.
中文翻译：
控制 DNA 杂交链反应
报道了一种使用杂交链反应 (HCR) 控制亚稳态 DNA 发夹寡聚化的新方法。通过在发夹双链体中引入碱基对错配来实现控制。错配修饰允许人们在动力学上区分起始事件与传播事件，导致 DNA 寡聚体长达 10 个单体，并将分散性从 2.5 提高到 1.3-1.6。重要的是，即使在连续两次扩链后，分散性仍不受影响，表明可以实现明确定义的嵌段共聚低聚物。作为概念验证，该技术随后应用于用突变绿色荧光蛋白功能化的发夹单体，以制备蛋白质寡聚物。综合起来，
更新日期：2020-05-01
中文翻译：
控制 DNA 杂交链反应
报道了一种使用杂交链反应 (HCR) 控制亚稳态 DNA 发夹寡聚化的新方法。通过在发夹双链体中引入碱基对错配来实现控制。错配修饰允许人们在动力学上区分起始事件与传播事件，导致 DNA 寡聚体长达 10 个单体，并将分散性从 2.5 提高到 1.3-1.6。重要的是，即使在连续两次扩链后，分散性仍不受影响，表明可以实现明确定义的嵌段共聚低聚物。作为概念验证，该技术随后应用于用突变绿色荧光蛋白功能化的发夹单体，以制备蛋白质寡聚物。综合起来，
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