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SHOOT MERISTEMLESS is Required for the Proper Internode Patterning and the Sepal Separation in Arabidopsis
Journal of Plant Biology ( IF 2.2 ) Pub Date : 2020-02-19 , DOI: 10.1007/s12374-020-09235-9 Sang-Kee Song , Young Bin Yun , Myeong Min Lee
Journal of Plant Biology ( IF 2.2 ) Pub Date : 2020-02-19 , DOI: 10.1007/s12374-020-09235-9 Sang-Kee Song , Young Bin Yun , Myeong Min Lee
SHOOT MERISTMLESS (STM) encoding a homeodomain protein plays a vital role in the specification of shoot meristem, the source of undifferentiated cells for the above-ground organ development in Arabidopsis thaliana. The roles of STM still remain to be elucidated as stm is deficient in the late organ development. Here, we have isolated a recessive filamentous gynoecium (fig)-1 displaying slightly reduced floral meristem and weak sepal fusion defect by introducing the 35S enhancer tag into the poltergeist-6. A truncated tag was localized at the 4628 base pairs (bps) upstream from the STM start codon in fig-1 led to the decrease in the STM transcripts. Two additional knocked-down mutant alleles, stm-p1 and stm-p3 harboring T-DNA insertions at 468 and 2225 bps upstream from the start codon, respectively displayed the pennywise (pny)-like clustered internode together with the sepal fusion defects. In stm-p1, the expression of KNOTTED-1 LIKE FROM A. THALIANA6 was enhanced and stm-p1 brevipedicelus-1 developed extremely compact internodes. The sepal fusion phenotypes of stm-p1 and stm-p3 were comparable to that of cup-shaped cotyledon1 (cuc1) and CUC1 expression was reduced in stm-p1. Together, these results suggest that STM functions genetically in association with PNY and CUC1 for the proper inflorescence development and the sepal separation, respectively.
中文翻译:
拟南芥中正确的节间构图和分隔需要拍摄地无种子
编码同源结构域蛋白的SHOOT MERISTMLESS(STM)在枝条分生组织的规格中起着至关重要的作用,枝条分生组织是拟南芥地上器官发育的未分化细胞的来源。由于stm在晚期器官发育中缺乏,因此STM的作用仍有待阐明。在这里,我们通过将35S增强子标签引入poltergeist - 6中,分离出了隐隐的丝状妇科(图)-1,其花分生组织略微减少,而萼片融合缺陷较弱。截短的标签位于4628个碱基对(bps)的上游。STM起始密码子用图- 1导致了下降STM成绩单。在起始密码子上游468和2225 bps处带有T-DNA插入的另外两个敲除突变等位基因stm - p1和stm - p3分别显示了似pennywise(pny)样的簇状节间以及隔膜融合缺陷。在stm - p1中,拟南芥(A. THALIANA6)的KNOTTED - 1样表达增强,stm - p1 brevipedicelus - 1增强。开发了非常紧凑的节点间。的萼片融合表型STM - P1和STM - P3均比得上杯-成形cotyledon1(cuc1)和CUC1表达降低在STM - P1。在一起,这些结果表明STM分别与PNY和CUC1遗传结合,分别进行适当的花序发育和萼片分离。
更新日期:2020-02-19
中文翻译:
拟南芥中正确的节间构图和分隔需要拍摄地无种子
编码同源结构域蛋白的SHOOT MERISTMLESS(STM)在枝条分生组织的规格中起着至关重要的作用,枝条分生组织是拟南芥地上器官发育的未分化细胞的来源。由于stm在晚期器官发育中缺乏,因此STM的作用仍有待阐明。在这里,我们通过将35S增强子标签引入poltergeist - 6中,分离出了隐隐的丝状妇科(图)-1,其花分生组织略微减少,而萼片融合缺陷较弱。截短的标签位于4628个碱基对(bps)的上游。STM起始密码子用图- 1导致了下降STM成绩单。在起始密码子上游468和2225 bps处带有T-DNA插入的另外两个敲除突变等位基因stm - p1和stm - p3分别显示了似pennywise(pny)样的簇状节间以及隔膜融合缺陷。在stm - p1中,拟南芥(A. THALIANA6)的KNOTTED - 1样表达增强,stm - p1 brevipedicelus - 1增强。开发了非常紧凑的节点间。的萼片融合表型STM - P1和STM - P3均比得上杯-成形cotyledon1(cuc1)和CUC1表达降低在STM - P1。在一起,这些结果表明STM分别与PNY和CUC1遗传结合,分别进行适当的花序发育和萼片分离。