Abstract Endophytes living inside plant tissues are known to be a rich source of unique bioactive compounds. In the current study, endophytic fungi were isolated from the leaves, stems and roots of five species of epiphytic orchids: Doritis pulcherrima, Dendrobiuma phyllum, Dendrobium anosmum, Ascocentrum curvifolium and Aerides falcata. In total, 52 endophytic isolates were obtained from the orchid samples, with the most (27) being obtained from leaf segments followed by roots and stems. All of the endophytic isolates were assessed for their potential role in producing extracellular enzymes, including amylase, protease, cellulase, pectinase and lipase on solid media. The results showed that 67%, 56%, 49%, 39% and 25% of the endophytic isolates had the ability to produce lipase, cellulase, pectinase, protease and amylase enzymes, respectively. One endophytic isolate, DapR 02, isolated from the root of Dendrobium aphyllum produced significantly more pectinase enzyme secretion with a ratio of the clear zone diameter to the colony diameter of 23. It was identified as Pseudopestalotiopsis theae using morphological characteristics and molecular analysis. This was the first report of the pectinase enzyme production potential of the endophyte Pseudopestalotiopsis theae. Therefore, an experiment was conducted to optimize the pectinase production in the DapR 02 isolate. High pectinase production of 1.524 U/mL was observed under culture conditions of 30 °C and pH 3.

中文翻译：

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泰国兰花内生真菌胞外酶活性的分离与筛选

摘要 众所周知，生活在植物组织内的内生菌是独特的生物活性化合物的丰富来源。在目前的研究中，从五种附生兰花的叶、茎和根中分离出内生真菌：Doritis pulcherrima、Dendrobium phyllum、Dendrobium anosmum、Ascocentrum curvifolium 和 Aerides falcata。总共从兰花样品中获得了 52 个内生分离株，其中大部分（27 个）来自叶段，其次是根和茎。评估了所有内生分离物在产生细胞外酶（包括在固体培养基上的淀粉酶、蛋白酶、纤维素酶、果胶酶和脂肪酶）方面的潜在作用。结果表明，67%、56%、49%、39%和25%的内生分离株分别具有产生脂肪酶、纤维素酶、果胶酶、蛋白酶和淀粉酶的能力。从 Dendrobium aphyllum 根中分离出的一种内生分离物 DapR 02 产生显着更多的果胶酶分泌，其透明带直径与菌落直径之比为 23。使用形态特征和分子分析将其鉴定为 Pseudopestalotiopsis theae。这是对内生菌 Pseudopestalotiopsis theae 果胶酶生产潜力的首次报道。因此，进行了一项实验以优化 DapR 02 分离株中的果胶酶生产。在 30 °C 和 pH 3 的培养条件下观察到 1.524 U/mL 的高果胶酶产量。使用形态特征和分子分析将其鉴定为 Pseudopestalotiopsis theae。这是对内生菌 Pseudopestalotiopsis theae 果胶酶生产潜力的首次报道。因此，进行了一项实验以优化 DapR 02 分离株中的果胶酶生产。在 30 °C 和 pH 3 的培养条件下观察到 1.524 U/mL 的高果胶酶产量。使用形态特征和分子分析将其鉴定为 Pseudopestalotiopsis theae。这是对内生菌 Pseudopestalotiopsis theae 果胶酶生产潜力的首次报道。因此，进行了一项实验以优化 DapR 02 分离株中的果胶酶生产。在 30 °C 和 pH 3 的培养条件下观察到 1.524 U/mL 的高果胶酶产量。