Enzymatic Oxidation of Butane to 2‐Butanol in a Bubble Column,ChemCatChem

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Enzymatic Oxidation of Butane to 2‐Butanol in a Bubble Column
ChemCatChem ( IF 3.8 ) Pub Date : 2020-04-28 , DOI: 10.1002/cctc.202000431
Frederic Perz ₁ , Sebastian Bormann ₂ , Roland Ulber ₃ , Miguel Alcalde ₄ , Paul Bubenheim ₁ , Frank Hollmann ₅ , Dirk Holtmann ₆ , Andreas Liese ₁

Affiliation

Unspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non‐activated C−H bonds using only hydrogen peroxide as a co‐substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2‐butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2‐butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions.

中文翻译：

鼓泡塔中丁烷的酶氧化成2-丁醇

非特异性过氧化酶由于仅使用过氧化氢作为共底物来催化未活化的CH键的羟基化的能力而引起了人们的极大兴趣。然而，到目前为止，制备方法的发展主要集中在使用液体底物的苄基羟基化上。在本文中，我们证明了过氧合酶在鼓泡塔反应器中将惰性气态底物丁烷羟基化为2-丁醇的应用。在200 mL规模下研究了过氧化氢进料速度和酶负载对产物形成，丁酮过氧化和催化效率的影响。该工艺可放大至2 L，并进行连续萃取。该设置可生产115 mmol的2-丁醇和70 mmol的丁酮，总总流通量（TTN）超过15。

更新日期：2020-04-28

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