当前位置:
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biochemical Characterization and Structural Analysis of a β-N-Acetylglucosaminidase from Paenibacillus barengoltzii for Efficient Production of N-Acetyl-d-glucosamine.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-05-11 , DOI: 10.1021/acs.jafc.9b08085 Yihao Liu 1 , Zhengqiang Jiang 2 , Junwen Ma 2 , Shuai Ma 3 , Qiaojuan Yan 1 , Shaoqing Yang 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-05-11 , DOI: 10.1021/acs.jafc.9b08085 Yihao Liu 1 , Zhengqiang Jiang 2 , Junwen Ma 2 , Shuai Ma 3 , Qiaojuan Yan 1 , Shaoqing Yang 2
Affiliation
![]() |
Bioproduction of N-acetyl-d-glucosamine (GlcNAc) from chitin, the second most abundant natural renewable polymer on earth, is of great value in which chitinolytic enzymes play key roles. In this study, a novel glycoside hydrolase family-18 β-N-acetylglucosaminidase (PbNag39) from Paenibacillus barengoltzii suitable for GlcNAc production was identified and biochemically characterized. It possessed a unique shallow catalytic groove (5.8 Å) as well as a smaller C-terminal domain (solvent-accessible surface area, 5.1 × 103 Å2) and exhibited strict substrate specificity toward N-acetyl chitooligosaccharides (COS) with GlcNAc as the sole product, showing a typical manner of action of β-N-acetylglucosaminidases. Thus, an environmentally friendly bioprocess for GlcNAc production from ball-milled powdery chitin by an enzyme cocktail reaction was further developed. By using the new route, the powdery chitin conversion rate increased from 23.3% (v/v) to 75.3% with a final GlcNAc content of 22.6 mg mL-1. The efficient and environmentally friendly bioprocess may have great application potential in GlcNAc production.
中文翻译:
来自巴氏芽孢杆菌的β-N-乙酰氨基葡萄糖苷酶的生化特征和结构分析,用于高效生产N-乙酰-d-氨基葡萄糖。
由几丁质(地球上第二大天然可再生聚合物)的生物生产N-乙酰基-d-葡萄糖胺(GlcNAc)具有巨大的价值,其中几丁质分解酶起着关键作用。在这项研究中,鉴定了适用于GlcNAc生产的来自巴氏芽孢杆菌的新颖的糖苷水解酶家族-18β-N-乙酰氨基葡糖苷酶(PbNag39),并对其进行了生化表征。它具有独特的浅催化槽(5.8Å)和较小的C末端结构域(溶剂可及的表面积5.1×103Å2),并以GlcNAc为唯一底物对N-乙酰基寡糖(COS)表现出严格的底物特异性。产物,显示出β-N-乙酰氨基葡萄糖苷酶的典型作用方式。从而,进一步开发了一种环境友好的生物工艺,该工艺通过酶鸡尾酒反应从球磨粉状甲壳素生产GlcNAc。通过使用新途径,粉末状几丁质转化率从23.3%(v / v)增加到75.3%,最终GlcNAc含量为22.6 mg mL-1。高效,环保的生物工艺可能在GlcNAc生产中具有巨大的应用潜力。
更新日期:2020-04-26
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
来自巴氏芽孢杆菌的β-N-乙酰氨基葡萄糖苷酶的生化特征和结构分析,用于高效生产N-乙酰-d-氨基葡萄糖。
由几丁质(地球上第二大天然可再生聚合物)的生物生产N-乙酰基-d-葡萄糖胺(GlcNAc)具有巨大的价值,其中几丁质分解酶起着关键作用。在这项研究中,鉴定了适用于GlcNAc生产的来自巴氏芽孢杆菌的新颖的糖苷水解酶家族-18β-N-乙酰氨基葡糖苷酶(PbNag39),并对其进行了生化表征。它具有独特的浅催化槽(5.8Å)和较小的C末端结构域(溶剂可及的表面积5.1×103Å2),并以GlcNAc为唯一底物对N-乙酰基寡糖(COS)表现出严格的底物特异性。产物,显示出β-N-乙酰氨基葡萄糖苷酶的典型作用方式。从而,进一步开发了一种环境友好的生物工艺,该工艺通过酶鸡尾酒反应从球磨粉状甲壳素生产GlcNAc。通过使用新途径,粉末状几丁质转化率从23.3%(v / v)增加到75.3%,最终GlcNAc含量为22.6 mg mL-1。高效,环保的生物工艺可能在GlcNAc生产中具有巨大的应用潜力。