High-efficiency dynamic sensing of biothiols in cancer cells with a fluorescent β-cyclodextrin supramolecular assembly,Chemical Science

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High-efficiency dynamic sensing of biothiols in cancer cells with a fluorescent β-cyclodextrin supramolecular assembly
Chemical Science ( IF 7.6 ) Pub Date : 2020-04-22 , DOI: 10.1039/d0sc00414f
Zhixue Liu ₁ , Weilei Zhou ₁ , Jingjing Li ₁ , Haoyang Zhang ₁ , Xianyin Dai ₁ , Yaohua Liu ₁ , Yu Liu ₁

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A unique fluorescent supramolecular assembly was constructed using coumarin-modified β-cyclodextrin as a reversible ratiometric probe and an adamantane-modified cyclic arginine–glycine–aspartate peptide as a cancer-targeting agent via host–guest inclusion complexation. Importantly, the coumarin-modified β-cyclodextrin not only showed higher sensitivity than the parent coumarin derivatives owing to the presence of numerous hydroxyl groups on the cyclodextrin but also provided a hydrophobic cavity for encapsulation of a cancer-targeting agent. The assembly showed a reversible and fast response to biothiols with a micromolar dissociation constant, as well as outstanding cancer cell permeability, which can be used for high-efficiency real-time monitoring of biothiols in cancer cells. This supramolecular assembly strategy endows the fluorescent probe with superior performance for dynamic sensing of biothiols.

中文翻译：

利用荧光 β-环糊精超分子组装体高效动态检测癌细胞中的生物硫醇

使用香豆素修饰的 β-环糊精作为可逆比例探针和金刚烷修饰的环状精氨酸-甘氨酸-天冬氨酸肽作为癌症靶向剂，通过主客体包合络合构建了独特的荧光超分子组装体。重要的是，由于环糊精上存在大量羟基，香豆素修饰的β-环糊精不仅表现出比母体香豆素衍生物更高的灵敏度，而且还提供了用于封装癌症靶向剂的疏水空腔。该组装体表现出对微摩尔解离常数的生物硫醇的可逆快速响应，以及出色的癌细胞通透性，可用于高效实时监测癌细胞中的生物硫醇。这种超分子组装策略赋予荧光探针在生物硫醇的动态传感方面具有优异的性能。

更新日期：2020-04-22

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