当前位置：
X-MOL 学术
›
ChemistrySelect
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Preparation of Curcubit[6]uril functionalized CuO Nanoparticles: A New Nanosensing Scheme Based on Fluorescence recovery after FRET for the Label Free Determination of Dopamine
ChemistrySelect ( IF 1.9 ) Pub Date : 2020-04-20 , DOI: 10.1002/slct.202000595 Mayada Qasem 1 , Riham El Kurdi 1 , Digambara Patra 1
ChemistrySelect ( IF 1.9 ) Pub Date : 2020-04-20 , DOI: 10.1002/slct.202000595 Mayada Qasem 1 , Riham El Kurdi 1 , Digambara Patra 1
Affiliation
|
In this manuscript, the aim is to functionalize CuO nanoparticles with the supramolecular host molecule, in this case curcubit[6]uril to have a host guest interaction between the CuO nanoparticles and probe (guest) molecules. Acridine Orange (AO) is applied as a guest molecule. Acridine orange binds with curcubit[6]uril via its carbonyl rims. In the presence of curcubit[6]uril conjugated CuO nanoparticles, the fluorescence intensity of AO is quenched by the CuO NPs through fluorescence resonance energy transfer phenomenon. However, dopamine adsorbs on the surface of CuO nanoparticles by getting incorporated into CB[6] host molecules and kicks out AO from curcubit[6]uril pocket, thus, discouraging fluorescence resonance energy transfer phenomenon and thus enhancing fluorescence intensity of AO by a factor of 3. This fluorescence recovery has been utilized to design a new detection system for dopamine. The assay, measured at fluorescence excitation and emission wavelengths of 480 nm and 520 nm respectively, works in the 0–40 μM concentration range of dopamine with 40 nM limit of detection. This method is not interfered by ascorbic acid, uric acid, glucose, tryptophan and acetaminophen. The proposed method provides a good recovery from synthetic samples and shows good stability.
中文翻译：
Curbit [6] uril功能化的CuO纳米粒子的制备：基于FRET后荧光回收的无标记多巴胺测定的新纳米传感方案
在本手稿中，目的是用超分子主体分子功能化CuO纳米粒子，在这种情况下，为葫芦[6] uril，使其在CuO纳米粒子和探针（客体）分子之间具有宿主客体相互作用。cr啶橙（AO）用作客体分子。cr啶橙通过其羰基边缘与葫芦[6]尿素结合。在存在Cubit [6] uril共轭的CuO纳米粒子的情况下，AO的荧光强度通过荧光共振能量转移现象被CuO NPs猝灭。然而，多巴胺通过掺入CB [6]宿主分子中而吸附在CuO纳米颗粒的表面上，并从葫芦[6] uril袋中踢出AO，从而阻止了荧光共振能量转移现象，从而将AO的荧光强度提高了一个因素。的3。这种荧光回收率已被用来设计一种新的多巴胺检测系统。该检测分别在480 nm和520 nm的荧光激发和发射波长下进行，在多巴胺的0–40μM浓度范围内进行，检测限为40 nM。该方法不受抗坏血酸，尿酸，葡萄糖，色氨酸和对乙酰氨基酚的干扰。所提出的方法可从合成样品中很好地回收并显示出良好的稳定性。
更新日期：2020-04-22
中文翻译：
Curbit [6] uril功能化的CuO纳米粒子的制备：基于FRET后荧光回收的无标记多巴胺测定的新纳米传感方案
在本手稿中，目的是用超分子主体分子功能化CuO纳米粒子，在这种情况下，为葫芦[6] uril，使其在CuO纳米粒子和探针（客体）分子之间具有宿主客体相互作用。cr啶橙（AO）用作客体分子。cr啶橙通过其羰基边缘与葫芦[6]尿素结合。在存在Cubit [6] uril共轭的CuO纳米粒子的情况下，AO的荧光强度通过荧光共振能量转移现象被CuO NPs猝灭。然而，多巴胺通过掺入CB [6]宿主分子中而吸附在CuO纳米颗粒的表面上，并从葫芦[6] uril袋中踢出AO，从而阻止了荧光共振能量转移现象，从而将AO的荧光强度提高了一个因素。的3。这种荧光回收率已被用来设计一种新的多巴胺检测系统。该检测分别在480 nm和520 nm的荧光激发和发射波长下进行，在多巴胺的0–40μM浓度范围内进行，检测限为40 nM。该方法不受抗坏血酸，尿酸，葡萄糖，色氨酸和对乙酰氨基酚的干扰。所提出的方法可从合成样品中很好地回收并显示出良好的稳定性。
京公网安备 11010802027423号