基于姜黄素-铜（II）集成体的荧光“打开”模式，可在原位和体内感应植物防御激素水杨酸。,Journal of Agricultural and Food Chemistry

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基于姜黄素-铜（II）集成体的荧光“打开”模式，可在原位和体内感应植物防御激素水杨酸。
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-20 , DOI: 10.1021/acs.jafc.0c01283
Chong Chen ₁ , Lin-Lin Yang ₁ , A-Ling Tang ₁ , Pei-Yi Wang ₁ , Rong Dong ₁ , Zhi-Bing Wu ₁ , Zhong Li ₂ , Song Yang _{1,

2}

Affiliation

水杨酸（SA）是一种重要的植物来源信号分子，能够启动全局转录重编程，以帮助植物获得系统获得性抗性（SAR）机制。因此，对SA的准确检测不仅将大大有助于了解植物的SAR，而且还有助于保护作物以及农业生产和食品供应的安全。但是，使用荧光探针检测SA对科学家来说是一个巨大的挑战，因为SA类似物会显着干扰检测结果。在本文中，我们首次报道了使用简单的天然姜黄素-Cu2 +整体在荧光“开启”模式的指导下选择性地和敏感地监测SA的原位和体内。首先用荧光法制备了姜黄素-Cu2 +的二元组合减少了由Cu2 +的顺磁性引起的荧光影响。进一步的研究表明，合理设计的杂交探针可以监测活细胞系中的SA。我们希望这一发现可以激发高性能SA探针的发现。减少了由Cu2 +的顺磁性引起的荧光影响。进一步的研究表明，合理设计的杂交探针可以监测活细胞系中的SA。我们期望这一发现可以激发高性能SA探针的发现。

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Curcumin-Cu(II) Ensemble-Based Fluorescence "Turn-On" Mode Sensing the Plant Defensive Hormone Salicylic Acid In Situ and In Vivo.

Salicylic acid (SA), a crucial, plant-derived signal molecule, is capable of launching global transcriptional reprogramming to assist plants in obtaining the systemic acquired resistance (SAR) mechanism. Thus, the accurate detection of SA will not only significantly contribute to the understanding of the plant SAR but also contribute to crop protection and to the security of the agricultural production and food supply. However, detection of SA using fluorescent probes is a great challenge for scientists, because SA analogues can significantly interfere with the detection results. Herein, we first reported using a simple, natural curcumin-Cu2+ ensemble to selectively and sensitively monitor SA in situ and in vivo, directed by a fluorescence "turn-on" mode. A binary combination curcumin-Cu2+ was first fabricated with a fluorescence "turn-off" pattern caused by the paramagnetic nature of Cu2+. Subsequently, a fluorescence "turn-on" response was performed for detecting SA accompanied by the formation of the ternary complex curcumin-Cu2+-SA due to the high affinity of SA toward Cu2+, which reduced the fluorescent impact caused by the paramagnetism of Cu2+. Further study revealed that the rationally designed hybrid probe could monitor SA in living cell lines. We anticipate that this finding can inspire the discovery of a high-performance SA probe.

更新日期：2020-04-24

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