建立朗格缪尔膜天平上用于粒子相互作用研究的合成体外肺表面活性剂模型。,Langmuir

当前位置： X-MOL 学术 › Langmuir › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

建立朗格缪尔膜天平上用于粒子相互作用研究的合成体外肺表面活性剂模型。
Langmuir ( IF 3.7 ) Pub Date : 2020-04-20 , DOI: 10.1021/acs.langmuir.9b03712
Julia J Schüer ₁ , Alexej Arndt ₁ , Christian Wölk _{2,

3} , Shashank R Pinnapireddy ₁ , Udo Bakowsky ₁

Affiliation

为了提供可用于预测颗粒与肺表面活性剂相互作用的稳健而经济的模型，本研究旨在寻找一种完美模仿天然肺表面活性剂特性的人工表面活性剂模型。表面活性剂模型应具有可再现性，鲁棒性，并能够预测肺表面活性剂与空气和水性部位的外在影响之间的相互作用。我们将三种合成模型与天然牛表面活性剂Alveofact进行了比较。通过在具有可移动屏障的Langmuir槽中的空气/水界面处铺展表面活性剂来模拟肺部状况。所有三种人工表面活性剂模型均显示出与Alveofact非常相似的特性。通过原子力显微镜对单层的可视化揭示了具有域形成的非常相似的结构。田中脂质混合物在先前的研究中已经在体外和体内显示出良好的结果。1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC）-1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）模型的表面压力等温线具有较大的构型，并显示出仿生排斥平台，指示有效的肺表面活性剂配方。同样，平衡扩散压力也相似。DPPC-1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸-1'-rac-甘油（POPG）在滞后区与Alveofact的相似性最大。解吸过程中弛豫实验的动力学常数表明，PCPG模型（30 mN / m）具有与Alveofact几乎相同的扩散和溶出值。作为概念证明，在所有三种表面活性剂模型的所有实验中，模型与PLGA纳米颗粒的相互作用均显示出令人鼓舞的结果。结果表明，模型中组件的选择在获得可再现的结果中起着至关重要的作用。所选模型可作为合成的体外肺模型用于进一步研究。

"点击查看英文标题和摘要"

Establishment of a Synthetic In Vitro Lung Surfactant Model for Particle Interaction Studies on a Langmuir Film Balance.

With the intention to provide a robust and economical model that can be used for predicting particle interactions with the pulmonary surfactant, this study was aimed to find an artificial surfactant model that perfectly mimics the properties of the natural pulmonary surfactant. A surfactant model should be reproducible, robust, and able to predict interactions between the pulmonary surfactant and exogenous influences from air and the aqueous site. We compared three synthetic models with the natural bovine surfactant Alveofact. The lung conditions were simulated by spreading the surfactants at the air/aqueous interface on a Langmuir trough with movable barriers. All three artificial surfactant models showed properties very similar to that of Alveofact. Visualization of the monolayers by atomic force microscopy revealed very similar structures with domain formation. The Tanaka lipid mixture has already shown good results in vitro and in vivo in previous studies. The 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)-1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) model has large conformations in the surface pressure isotherms and showed a biomimetic exclusion plateau, indicative of an effective lung surfactant formulation. Also, the equilibrium spreading pressure was similar. DPPC-1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-1'-rac-glycerol (POPG) had the greatest similarities with Alveofact in the hysteresis areas. The kinetic constants of the relaxation experiments during desorption showed that the PCPG model (at 30 mN/m) had almost identical diffusion and dissolution values as Alveofact. As a proof of concept, the interaction of the models with PLGA nanoparticles showed promising results in all experiments for all the three surfactant models. The results show that the choice of components in a model play a crucial role in obtaining reproducible results. The selected models can be used for further studies as synthetic in vitro lung models.

更新日期：2020-04-20

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南