简短报告：循环 microRNA 与日裔美国人 10 年来的糖尿病发生有关。,Scientific Reports

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简短报告：循环 microRNA 与日裔美国人 10 年来的糖尿病发生有关。
Scientific Reports ( IF 3.8 ) Pub Date : 2020-04-16 , DOI: 10.1038/s41598-020-63606-3
Pandora L Wander _{1,

2} , Daniel A Enquobahrie ₃ , Theo K Bammler ₄ , Sengkeo Srinouanprachanh ₄ , James MacDonald ₄ , Steven E Kahn _{1,

2} , Donna Leonetti ₅ , Wilfred Y Fujimoto ₁ , Edward J Boyko _{1,

2}

Affiliation

表观遗传变化早于糖尿病的发生多年，为其发病机制提供了线索。我们探讨了表观遗传标记、循环 microRNA (miRNA) 是否与日裔美国人的糖尿病发生有关。我们进行了一项试点研究（n = 10），使用日裔美国人社区糖尿病研究（一项糖尿病危险因素的观察性研究）中年龄和性别匹配的参与者的血浆，这些参与者患有或未患糖尿病。使用基线收集的样本进行 miRNA 的提取和高通量测序。回归模型适合比较在 10 年随访中发生或未发生糖尿病的个体中的循环 miRNA (N = 1640)。参与者的基线平均年龄为 51.7 岁；60%是男性。我们鉴定出患有糖尿病的个体与未患糖尿病的个体相比，存在不同水平的 36 个 miRNA（10 个较高和 26 个较低）（log2 倍变化≥1.25，错误发现率≤5%）。其中包括在骨骼肌胰岛素代谢中具有功能的 miRNA（miR-106b 和 miR-20b-5p）以及在骨骼肌胰岛素代谢和内分泌胰腺细胞周期调节中具有功能的 miRNA（miR-15a 和 miR-17）。经过 10 年的随访，循环 miRNA 与日裔美国人随后患糖尿病有关。结果是初步的。大规模 miRNA 测序研究可以帮助我们了解糖尿病发病机制，并开发基于基因表达调控的针对糖尿病的疗法。

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Short Report: Circulating microRNAs are associated with incident diabetes over 10 years in Japanese Americans.

Epigenetic changes precede the development of diabetes by many years, providing clues to its pathogenesis. We explored whether the epigenetic markers, circulating microRNAs (miRNAs), were associated with incident diabetes in Japanese Americans. We conducted a pilot study (n = 10) using plasma from age- and sex-matched participants who did or did not develop diabetes in the Japanese American Community Diabetes Study, an observational study of diabetes risk factors. Extraction and high-throughput sequencing of miRNAs were performed using samples collected at baseline. Regression models were fit comparing circulating miRNAs (N = 1640) among individuals who did or did not develop incident diabetes at 10-year follow-up. Participants averaged 51.7 years of age at baseline; 60% were male. We identified 36 miRNAs present at different (10 higher and 26 lower) levels in individuals who developed diabetes compared to those who did not (log2fold change ≥1.25 and false discovery rate ≤5%). These included miRNAs with functions in skeletal muscle insulin metabolism (miR-106b and miR-20b-5p) and miRNAs with functions in both skeletal muscle insulin metabolism and cell cycle regulation in endocrine pancreas (miR-15a and miR-17). Circulating miRNAs were associated with subsequent development of diabetes among Japanese Americans over 10 years of follow-up. Results are preliminary. Large-scale miRNA sequencing studies could inform our understanding of diabetes pathogenesis and development of therapies, based on gene expression regulation, that target diabetes.

更新日期：2020-04-24

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