<br>布基纳法索与甘薯病毒病相关的病毒种类特征,Plant Pathology

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布基纳法索与甘薯病毒病相关的病毒种类特征
Plant Pathology ( IF 2.3 ) Pub Date : 2020-04-30 , DOI: 10.1111/ppa.13190
Ezechiel B Tibiri _{1,

2,

3,

4} , Justin S Pita ₅ , Fidèle Tiendrébéogo _{1,

3} , Martine Bangratz _{3,

6} , James B Néya _{1,

3} , Christophe Brugidou _{3,

6} , Koussao Somé _{2,

3} , Nicolas Barro ₄

Affiliation

摘要撒哈拉以南非洲的甘薯（Ipomoea batatas）生产受到几种相互作用的病毒引起的病毒病的严重影响，包括甘薯羽状斑驳病毒（SPFMV）、甘薯褪绿矮化病毒（SPCSV）和甘薯曲叶病毒（SPCSV）。 SPLCV）。然而，在大多数非洲国家，甘薯病毒症状的病因学很少确定。在这里，我们的目的是调查和描述布基纳法索甘薯病毒的发病率。我们在布基纳法索的 18 个地区进行了全国范围的调查，从 80 个田地中收集了 600 株有或没有症状的植物。使用硝酸纤维素膜 ELISA、PCR 和逆转录 PCR 鉴定病毒株。选取 50 株有症状的植物，每株选取 3 个接穗，将其嫁接到健康的山番薯上，然后对 150 个记录的样品进行血清学和分子检测。检测到三种病毒：24% 的样本 SPFMV 呈阳性，18% 的样本呈 SPLCV 阳性，2% 的样本呈 SPCSV 阳性。在所有诊断测试中，40% 的植物样本病毒呈阴性。 16% 的样本中发现了混合感染。获得了部分序列，其中13个匹配SPFMV，1个匹配SPLCV，1个匹配SPCSV。所有已鉴定的 SPFMV 分离株均属于系统群 B 或 A-II。 SPCSV 阳性分离株的外壳蛋白与 SPCSV 西非有 98% 的基因序列同源性。 Begomovirus 阳性分离株聚集在 SPLCV-美国。布基纳法索对甘薯病毒性疾病的首次研究表明该疾病广泛存在，并表明需要进一步进行流行病学调查、以抗病毒品种为重点的育种计划以及改进耕作方法以控制疾病传播。

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Characterisation of virus species associated with sweetpotato virus diseases in Burkina Faso

Abstract Sweetpotato (Ipomoea batatas) production in sub‐Saharan Africa is severely affected by viral diseases caused by several interacting viruses, including sweet potato feathery mottle virus (SPFMV), sweet potato chlorotic stunt virus (SPCSV), and sweet potato leaf curl virus (SPLCV). However, the aetiology of viral symptoms on sweetpotato is rarely established in most countries in Africa. Here, we aimed to investigate and characterize the incidence of sweetpotato viruses in Burkina Faso. We performed a countrywide survey in 18 districts of Burkina Faso and collected 600 plants, with and without symptoms, from 80 fields. Viral strains were identified using nitrocellulose membrane‐ELISA, PCR, and reverse transcription‐PCR. Three scions from each of 50 selected plants with symptoms were grafted to healthy Ipomoea setosa and then serological and molecular tests were performed on the 150 recorded samples. Three viruses were detected: 24% of samples were positive for SPFMV, 18% for SPLCV, and 2% for SPCSV. Across all diagnostic tests, 40% of all plant samples were virus‐negative. Coinfections were found in 16% of samples. Partial sequences were obtained, including 13 that matched SPFMV, one that matched SPLCV, and one that matched SPCSV. All identified SPFMV isolates belonged to either phylogroup B or A‐II. The SPCSV‐positive isolates had 98% gene sequence homology with SPCSV‐West Africa for the coat protein. Begomovirus‐positive isolates clustered with SPLCV‐United States. This first study of sweetpotato viral diseases in Burkina Faso indicates widespread occurrence and suggests a need for further epidemiological investigations, breeding programmes focused on virus‐resistant varieties, and improved farming practices to control disease spread.

更新日期：2020-04-30

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