Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in Arabidopsis.,Nature Communications

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Transcriptional repression of GIF1 by the KIX-PPD-MYC repressor complex controls seed size in Arabidopsis.
Nature Communications ( IF 14.7 ) Pub Date : 2020-04-15 , DOI: 10.1038/s41467-020-15603-3
Zupei Liu _{1,

2} , Na Li ₁ , Yueying Zhang _{1,

2} , Yunhai Li _{1,

2}

Affiliation

Seed size is a key agronomic trait that greatly determines plant yield. Elucidating the molecular mechanism underlying seed size regulation is also an important question in developmental biology. Here, we show that the KIX-PPD-MYC-GIF1 pathway plays a crucial role in seed size control in Arabidopsis thaliana. Disruption of KIX8/9 and PPD1/2 causes large seeds due to increased cell proliferation and cell elongation in the integuments. KIX8/9 and PPD1/2 interact with transcription factors MYC3/4 to form the KIX-PPD-MYC complex in Arabidopsis. The KIX-PPD-MYC complex associates with the typical G-box sequence in the promoter of GRF-INTERACTING FACTOR 1 (GIF1), which promotes seed growth, and represses its expression. Genetic analyses support that KIX8/9, PPD1/2, MYC3/4, and GIF1 function in a common pathway to control seed size. Thus, our results reveal a genetic and molecular mechanism by which the transcription factors MYC3/4 recruit KIX8/9 and PPD1/2 to the promoter of GIF1 and repress its expression, thereby determining seed size in Arabidopsis.

中文翻译：

KIX-PPD-MYC阻遏物复合物对GIF1的转录抑制可控制拟南芥中的种子大小。

种子大小是决定农作物产量的关键农艺性状。阐明种子大小调控的分子机制也是发育生物学中的重要问题。在这里，我们表明，KIX-PPD-MYC-GIF1途径在拟南芥种子大小控制中起着至关重要的作用。KIX8 / 9和PPD1 / 2的破坏会导致较大的种子，这是由于其外皮中的细胞增殖和细胞伸长增加。KIX8 / 9和PPD1 / 2与转录因子MYC3 / 4相互作用，在拟南芥中形成KIX-PPD-MYC复合体。KIX-PPD-MYC复合物与GRF相互作用因子1（GIF1）启动子中的典型G-box序列相关，后者可促进种子生长并抑制其表达。遗传分析支持KIX8 / 9，PPD1 / 2，MYC3 / 4和GIF1在控制种子大小的通用途径中发挥作用。从而，

更新日期：2020-04-24

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