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Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells.
Chemosphere ( IF 8.1 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.chemosphere.2020.126788 Qiangqiang Wang 1 , Jialei Bai 2 , Baoan Ning 2 , Longxing Fan 2 , Tieqiang Sun 2 , Yanjun Fang 2 , Jin Wu 2 , Shuang Li 2 , Chenhui Duan 1 , Yingchun Zhang 2 , Jun Liang 3 , Zhixian Gao 2
Chemosphere ( IF 8.1 ) Pub Date : 2020-04-14 , DOI: 10.1016/j.chemosphere.2020.126788 Qiangqiang Wang 1 , Jialei Bai 2 , Baoan Ning 2 , Longxing Fan 2 , Tieqiang Sun 2 , Yanjun Fang 2 , Jin Wu 2 , Shuang Li 2 , Chenhui Duan 1 , Yingchun Zhang 2 , Jun Liang 3 , Zhixian Gao 2
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Microplastics are abundant in oceans, lakes, soils and even air, and can pose potential threats to human health through food or respiratory intake. Moreover, microplastics have synergistic toxicity to the body after absorbing organic pollutants. In this study, laser scanning confocal microscope and flow cytometry were used to observe the intake of colonic cancer Caco-2 cells to polystyrene plastic with five different particlesizes (300 nm, 500 nm, 1 μm, 3 μm, 6 μm). The uptake rates of microplastics with different particle sizes were 73%, 71%, 49%, 43%, and 30%, respectively. Then, High Performance Liquid Chromatography (HPLC) was used to analyze the adsorption differences of polystyrene plastic with different particle sizes to bisphenol A (BPA). Finally, the proliferation toxicity of polystyrene microplastics with different particle sizeson Caco-2 cells before and after adsorption of BPA was compared. MTT experiments confirmed that microplastics caused an increase in cytotoxicity. This result may be related to increased cellular oxidative stress and mitochondrial depolarization. This hypothesis has been confirmed in reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) assays because nanoscale microplastics cause a large amount of ROS on Caco-2 cells after microplastic exposure, and micron-scale microplastics cause a significant decrease in MMP. At the same time, nanoscale microplastics can cause further depolarization of mitochondria due to their large specific surface area adsorption of BPA, which leads to enhanced cytotoxicity of microplastics after BPA adsorption. The results of this study are of great significance in the evaluation of the safety of microplastics in the human body.
中文翻译：
双酚A和纳米级和微米级聚苯乙烯塑料暴露对人Caco-2细胞颗粒摄取和毒性的影响。
微塑料在海洋，湖泊，土壤甚至空气中含量很高，并可能通过食物或呼吸摄入对人类健康构成潜在威胁。此外，微塑料在吸收有机污染物后对人体具有协同毒性。在这项研究中，使用激光扫描共聚焦显微镜和流式细胞术来观察结肠癌Caco-2细胞摄入具有五种不同粒径（300 nm，500 nm，1μm，3μm，6μm）的聚苯乙烯塑料的情况。不同粒径的微塑料的吸收率分别为73％，71％，49％，43％和30％。然后，采用高效液相色谱法（HPLC）分析了粒径不同的聚苯乙烯塑料对双酚A（BPA）的吸附差异。最后，比较了不同粒径的聚苯乙烯微塑料在Caco-2细胞中吸附BPA前后的增殖毒性。MTT实验证实，微塑料导致细胞毒性增加。该结果可能与细胞氧化应激增加和线粒体去极化有关。此假设已在活性氧（ROS）和线粒体膜电位（MMP）分析中得到证实，因为纳米级微塑料在暴露于塑料后会在Caco-2细胞上引起大量ROS，而微米级微塑料会导致MMP显着降低。同时，纳米级微塑料由于其对BPA的大比表面积吸附，可导致线粒体进一步去极化，从而导致BPA吸附后微塑料的细胞毒性增强。
更新日期：2020-04-20
中文翻译：
双酚A和纳米级和微米级聚苯乙烯塑料暴露对人Caco-2细胞颗粒摄取和毒性的影响。
微塑料在海洋，湖泊，土壤甚至空气中含量很高，并可能通过食物或呼吸摄入对人类健康构成潜在威胁。此外，微塑料在吸收有机污染物后对人体具有协同毒性。在这项研究中，使用激光扫描共聚焦显微镜和流式细胞术来观察结肠癌Caco-2细胞摄入具有五种不同粒径（300 nm，500 nm，1μm，3μm，6μm）的聚苯乙烯塑料的情况。不同粒径的微塑料的吸收率分别为73％，71％，49％，43％和30％。然后，采用高效液相色谱法（HPLC）分析了粒径不同的聚苯乙烯塑料对双酚A（BPA）的吸附差异。最后，比较了不同粒径的聚苯乙烯微塑料在Caco-2细胞中吸附BPA前后的增殖毒性。MTT实验证实，微塑料导致细胞毒性增加。该结果可能与细胞氧化应激增加和线粒体去极化有关。此假设已在活性氧（ROS）和线粒体膜电位（MMP）分析中得到证实，因为纳米级微塑料在暴露于塑料后会在Caco-2细胞上引起大量ROS，而微米级微塑料会导致MMP显着降低。同时，纳米级微塑料由于其对BPA的大比表面积吸附，可导致线粒体进一步去极化，从而导致BPA吸附后微塑料的细胞毒性增强。
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