Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.,ACS Nano

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Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
ACS Nano ( IF 15.8 ) Pub Date : 2020-04-15 , DOI: 10.1021/acsnano.0c02823
Giwan Seo _{1,

2} , Geonhee Lee ₃ , Mi Jeong Kim _{1,

2} , Seung-Hwa Baek _{2,

4} , Minsuk Choi ₂ , Keun Bon Ku ₂ , Chang-Seop Lee _{5,

6} , Sangmi Jun _{2,

7} , Daeui Park _{2,

4} , Hong Gi Kim ₂ , Seong-Jun Kim ₂ , Jeong-O Lee ₃ , Bum Tae Kim ₂ , Edmond Changkyun Park _{1,

2,

8} , Seung Il Kim _{1,

2,

8}

Affiliation

Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.

中文翻译：

使用基于场效应晶体管的生物传感器快速检测人鼻咽拭子样本中的 COVID-19 致病病毒 (SARS-CoV-2)。

2019冠状病毒病（COVID-19）是由严重急性呼吸综合征冠状病毒2（SARS-CoV-2，以前称为2019-nCoV）引起的一种新出现的人类传染病。鉴于人类感染率迅速上升，世界卫生组织 (WHO) 将新型冠状病毒肺炎 (COVID-19) 疫情列为大流行病。由于尚无针对 COVID-19 的特效药物或疫苗，因此早期诊断和管理对于遏制疫情至关重要。在这里，我们报告了一种基于场效应晶体管（FET）的生物传感装置，用于检测临床样本中的 SARS-CoV-2。该传感器是通过在 FET 的石墨烯片上涂上针对 SARS-CoV-2 刺突蛋白的特异性抗体而制成的。该传感器的性能是使用来自 COVID-19 患者的抗原蛋白、培养病毒和鼻咽拭子样本来确定的。我们的 FET 装置可以检测磷酸盐缓冲盐水和 100 fg/mL 临床转运介质中浓度为 1 fg/mL 的 SARS-CoV-2 刺突蛋白。此外，FET 传感器成功检测了培养基中的 SARS-CoV-2（检测限 [LOD]：1.6 × 101 pfu/mL）和临床样本（LOD：2.42 × 102 拷贝/mL）。因此，我们成功制造了一种有前景的 SARS-CoV-2 FET 生物传感器；我们的设备是一种针对 COVID-19 的高度敏感的免疫学诊断方法，无需样品预处理或标记。

更新日期：2020-04-15

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