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Lipoprotein Lpp regulates the mechanical properties of the E. coli cell envelope.
Nature Communications ( IF 14.7 ) Pub Date : 2020-04-14 , DOI: 10.1038/s41467-020-15489-1
Marion Mathelié-Guinlet 1 , Abir T Asmar 2 , Jean-François Collet 2, 3 , Yves F Dufrêne 1, 3
Affiliation  

The mechanical properties of the cell envelope in Gram-negative bacteria are controlled by the peptidoglycan, the outer membrane, and the proteins interacting with both layers. In Escherichia coli, the lipoprotein Lpp provides the only covalent crosslink between the outer membrane and the peptidoglycan. Here, we use single-cell atomic force microscopy and genetically engineered strains to study the contribution of Lpp to cell envelope mechanics. We show that Lpp contributes to cell envelope stiffness in two ways: by covalently connecting the outer membrane to the peptidoglycan, and by controlling the width of the periplasmic space. Furthermore, mutations affecting Lpp function substantially increase bacterial susceptibility to the antibiotic vancomycin, indicating that Lpp-dependent effects can affect antibacterial drug efficacy.

中文翻译:

脂蛋白Lpp调节大肠杆菌细胞包膜的机械性能。

革兰氏阴性细菌中细胞膜的机械特性由肽聚糖,外膜和与两层相互作用的蛋白质控制。在大肠杆菌中,脂蛋白Lpp在外膜和肽聚糖之间提供唯一的共价交联。在这里,我们使用单细胞原子力显微镜和基因工程菌株研究Lpp对细胞包膜力学的贡献。我们表明,Lpp通过两种方式促进细胞包膜的刚度:通过共价将外膜与肽聚糖连接,以及通过控制周质空间的宽度。此外,影响Lpp功能的突变会大大增加细菌对抗生素万古霉素的敏感性,这表明Lpp依赖性作用会影响抗菌药物的功效。
更新日期:2020-04-24
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