Stable and Reusable Light-Responsive Reduced Covalent Organic Framework (COF-300-AR) as a Oxidase-Mimicking Catalyst for GSH Detection in Cell Lysate.,ACS Applied Materials & Interfaces

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Stable and Reusable Light-Responsive Reduced Covalent Organic Framework (COF-300-AR) as a Oxidase-Mimicking Catalyst for GSH Detection in Cell Lysate.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-04-22 , DOI: 10.1021/acsami.0c01763
Peng Jin ₁ , Xiaoying Niu ₁ , Fang Zhang ₁ , Kui Dong ₁ , Hongxia Dai ₁ , Huige Zhang ₁ , Weifeng Wang ₂ , Hongli Chen ₁ , Xingguo Chen ₁

Affiliation

Covalent organic frameworks (COFs), as one of the most significant members of the porous organic frameworks, have been well used in the photocatalysis owing to their outspread π-conjugated framework, high crystallinity and regular pore structure. Herein, after reducing the labile imine-linked COF-300 to the more stable amine-linked COF-300-AR, we for the first time demonstrated that COF-300-AR was the light-responsive oxidase mimic. COF-300-AR exhibited excellent oxidase-mimicking activity under purple light stimulation (λ = 400 nm), which can catalyze the oxidation of classical substrates such as 3,3',5,5'-tetramethylbenzydine (TMB) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by the formation of •OH and O2•- free radicals in the presence of dissolved oxygen. The COF-300-AR oxidase mimic has outstanding advantages of easy light control, high stability, good reusability, and highly catalytic oxidation capacity and has been applied to detect glutathione (GSH) levels in HL60 cells with good selectivity and high sensitivity. This study will broaden the sensing applications of COFs and offer a promising build block for the construction of artificial enzymes.

中文翻译：

稳定和可重复使用的光响应性还原共价有机骨架（COF-300-AR），作为氧化酶模拟催化剂用于细胞裂解液中GSH的检测。

共价有机骨架（COFs）作为多孔有机骨架的最重要成员之一，由于其扩展的π共轭骨架，高结晶度和规则的孔结构，已被广泛用于光催化中。在此，在将不稳定的亚胺连接的COF-300还原为更稳定的胺连接的COF-300-AR之后，我们首次证明了COF-300-AR是光响应性氧化酶模拟物。COF-300-AR在紫色光（λ= 400 nm）刺激下表现出出色的模仿氧化酶的活性，可催化3,3'，5,5'-四甲基联苯胺（TMB）和2,2等经典底物的氧化通过在溶解氧存在下形成•OH和O2•-自由基形成'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）。COF-300-AR氧化酶模拟物具有易于控制光，高稳定性，良好的可重复使用性和高催化氧化能力的突出优点，并已被用于检测HL60细胞中的谷胱甘肽（GSH）水平，具有良好的选择性和高灵敏度。这项研究将拓宽COF的传感应用，并为构建人造酶提供有希望的基础。

更新日期：2020-04-14

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