A TaqMan probe based real-time PCR for the detection of Decapod iridescent virus 1.,Journal of Invertebrate Pathology

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A TaqMan probe based real-time PCR for the detection of Decapod iridescent virus 1.
Journal of Invertebrate Pathology ( IF 3.6 ) Pub Date : 2020-04-03 , DOI: 10.1016/j.jip.2020.107367
Liang Qiu ₁ , Xing Chen ₁ , Xiao-Meng Guo ₂ , Wen Gao ₃ , Ruo-Heng Zhao ₁ , Qing-Li Zhang ₁ , Bing Yang ₁ , Jie Huang ₄

Affiliation

Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; Shanghai Ocean University, Shanghai 201306, China.

Decapod iridescent virus 1 (DIV1) was proven to be the aetiological agent of a disease causing mass die-offs of shrimp, prawn and crayfish. The specific purpose of this study was to develop a new sensitive real-time PCR method for the specific detection of DIV1. A pair of primers that amplify a 142 bp fragment and a TaqMan probe were selected for the major capsid protein gene of DIV1. They were shown to be specific for DIV1 and did not react with other common shrimp pathogens or healthy shrimp DNA. The method could detect as virus levels as low as 1.2 copies of DIV1 plasmid DNA.

中文翻译：

基于TaqMan探针的实时PCR用于检测十足类虹彩病毒1。

十足形虹彩病毒1（DIV1）被证明是引起虾，虾和小龙虾大量死亡的疾病的病原体。这项研究的特定目的是开发一种新的灵敏的实时PCR方法，用于DIV1的特异性检测。选择一对扩增142 bp片段的引物和一个TaqMan探针作为DIV1的主要衣壳蛋白基因。它们被证明对DIV1具有特异性，并且不与其他常见的虾病原体或健康的虾DNA反应。该方法可检测低至1.2份DIV1质粒DNA的病毒水平。

更新日期：2020-04-21

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