Clove is one of the most popular aromatic and medicinal plants used due to its strong bioactivity. Hydroalcoholic extract of the plants also named as tincture is a popular pharmaceutical form of the medicinal plants. The current study aims to determine the optimum production conditions of clove tincture in terms of the processing variables. For this purpose, response surface methodology was applied and liquid/solid ratio (X₁) and ethanol concentration (X₂) were selected as processing variables. As response, some antioxidant and antiradical capacity tests were conducted in addition to the concentration of photochemical constituents. Analysis of variance (ANOVA) showed that the both variables had a significant influence on both antioxidant and antiradical activities of the samples. Total phenolic and flavonoid contents of the tincture samples were in the range of 13.81–23.84 mg GAE/mL and 3.64–7.83 mg QE/mL, respectively while the ABTS^·+ radical scavenging activity and ferric reducing antioxidant activity (FRAA) values ranged from 44.78 to 174.70 μg Trolox/g sample and 62.77 to 87.71 mg AAE/L, respectively. Multiple response optimization indicated that the optimum values for the production of tincture were obtained for X₁ = 4.03 mL/g sample and X₂ = 53.2 % ethanol concentration. Our results indicated that the clove tincture having high antioxidant and antiradical performance could be produced at optimized conditions determined by response surface methodology.

丁香由于具有很强的生物活性，是最受欢迎的芳香和药用植物之一。植物的水醇提取物也被称为tin剂，是药用植物的流行药物形式。当前的研究旨在根据加工变量确定丁香cture的最佳生产条件。为此，应用了响应面分析法，液/固比（X ₁）和乙醇浓度（X ₂选择）作为处理变量。作为响应，除了光化学成分的浓度外，还进行了一些抗氧化剂和抗自由基能力测试。方差分析（ANOVA）表明，这两个变量对样品的抗氧化和抗自由基活性均具有显着影响。cture样品中的总酚和类黄酮含量分别在13.81–23.84 mg GAE / mL和3.64–7.83 mg QE / mL范围内，而ABTS ^·+自由基清除活性和三价铁还原抗氧化剂活性（FRAA）的范围为Trolox / g样品分别为44.78至174.70μg和AAE / L为62.77至87.71 mg。多重响应优化表明，X ₁获得了tin剂的最佳值。 ＝ 4.03mL / g样品，X ₂  ＝ 53.2％乙醇浓度。我们的结果表明，具有较高抗氧化剂和抗自由基性能的丁香tin剂可以在通过响应面法确定的最佳条件下生产。