双功能磁性纳米花：高效的固定化青霉素G酰基转移酶的载体,Journal of the Chinese Chemical Society

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双功能磁性纳米花：高效的固定化青霉素G酰基转移酶的载体
Journal of the Chinese Chemical Society ( IF 1.6 ) Pub Date : 2020-04-12 , DOI: 10.1002/jccs.201900559
Zheng Zhaoyu ₁ , Xue Ping ₁ , Shi Keren ₁ , Zhang Weiwei ₁ , Hu Chunmiao ₁ , Li Peng ₁

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通过扫描电子显微镜，透射电子显微镜（TEM），振动样品表征了具有环氧基和亲水性邻苯二酚以及能够与青霉素G酰基转移酶（PGA）共价偶联的邻苯二酚基的新型双功能磁性纳米花（DMNF）。磁力计，N ₂吸附等。研究表明，DMNF具有纳米片的“分层花瓣”结构，比饱和磁化强度为39.7 emu / g，平均孔径为25.4 nm，比表面积为17.28 m ²/G。固定在DMNF上的PGA催化酶负载106 mg / g的青霉素G钾的水解，其表观活性达到2,667 U / g，这得益于磁性DMNF的“分层花瓣”和大孔结构。高酶负载和底物分子快速扩散到固定的PGA上反应。重复使用10个循环后，固定化PGA的表观活性可以保持2,408 U / g（超过其初始活性的90％）。磁性固定的PGA由于PGA的氨基与DMNF上的环氧基，邻苯二酚和邻苯二酚基的反应性基团的共价相互作用，使酶分子在载体之间共价偶联，从而显示出优异的操作稳定性。此外，

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Di‐functional magnetic nanoflowers: A highly efficient support for immobilizing penicillin G acylase

The novel di‐functional magnetic nanoflowers (DMNF) which had both epoxy groups and hydrophilic catechol as well as phthaloquinone groups capable of covalently coupling of penicillin G acylase (PGA) were characterized by scanning electron microscopy, transmission electron microscope (TEM), vibrating sample magnetometer, N₂ adsorption, and so on. The studies showed that DMNF possessed “hierarchical petal” structure of nanosheets had specific saturation magnetization of 39.7 emu/g and average pore diameter of 25.4 nm as well as specific surface area of 17.28 m²/g. For hydrolysis of penicillin G potassium catalyzed by the PGA immobilized on DMNF with enzyme loading of 106 mg/g‐support, its apparent activity reached 2,667 U/g, which benefited from the “hierarchical petal” and large pore structure of the magnetic DMNF leading to high enzyme loading and fast diffusion of substrate molecules to the immobilized PGA to reaction. The apparent activity of the immobilized PGA could keep 2,408 U/g (above 90% of its initial activity) after repeating use for 10 cycles. The magnetic immobilized PGA exhibited excellent operational stability due to covalently coupling of the enzyme molecules between the support by covalent interaction of the amino groups of PGA and the reactive groups of epoxy, catechol, and phthaloquinone groups on DMNF. Furthermore, the PGA displayed good acid and alkaline resistance as well as thermal stability by immobilization using DMNF.

更新日期：2020-04-12

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